Human pre-valvular endocardial cells derived from pluripotent stem cells recapitulate cardiac pathophysiological valvulogenesis

Tui Neri; Emilye Hiriart; Patrick P. van Vliet; Emilie Faure; Russell A. Norris; Batoul Farhat; Bernd Jagla; Julie Lefrancois; Yukiko Sugi; Thomas Moore-Morris; Stéphane Zaffran; Randolph S. Faustino; Alexander C. Zambon; Jean Pierre Desvignes; David Salgado; Robert A. Levine; Jose Luis de la Pompa; André Terzic; Sylvia M. Evans; Roger Markwald; Michel Pucéat

doi:10.1038/s41467-019-09459-5

Human pre-valvular endocardial cells derived from pluripotent stem cells recapitulate cardiac pathophysiological valvulogenesis

Tui Neri, Emilye Hiriart, Patrick P. van Vliet, Emilie Faure, Russell A. Norris, Batoul Farhat, Bernd Jagla, Julie Lefrancois, Yukiko Sugi, Thomas Moore-Morris, Stéphane Zaffran, Randolph S. Faustino, Alexander C. Zambon, Jean Pierre Desvignes, David Salgado, Robert A. Levine, Jose Luis de la Pompa, André Terzic, Sylvia M. Evans, Roger MarkwaldMichel Pucéat

Cardiovascular Medicine

Research output: Contribution to journal › Article › peer-review

16 Scopus citations

Abstract

Genetically modified mice have advanced our understanding of valve development and disease. Yet, human pathophysiological valvulogenesis remains poorly understood. Here we report that, by combining single cell sequencing and in vivo approaches, a population of human pre-valvular endocardial cells (HPVCs) can be derived from pluripotent stem cells. HPVCs express gene patterns conforming to the E9.0 mouse atrio-ventricular canal (AVC) endocardium signature. HPVCs treated with BMP2, cultured on mouse AVC cushions, or transplanted into the AVC of embryonic mouse hearts, undergo endothelial-to-mesenchymal transition and express markers of valve interstitial cells of different valvular layers, demonstrating cell specificity. Extending this model to patient-specific induced pluripotent stem cells recapitulates features of mitral valve prolapse and identified dysregulation of the SHH pathway. Concurrently increased ECM secretion can be rescued by SHH inhibition, thus providing a putative therapeutic target. In summary, we report a human cell model of valvulogenesis that faithfully recapitulates valve disease in a dish.

Original language	English (US)
Article number	1929
Journal	Nature communications
Volume	10
Issue number	1
DOIs	https://doi.org/10.1038/s41467-019-09459-5
State	Published - Dec 1 2019

ASJC Scopus subject areas

Chemistry(all)
Biochemistry, Genetics and Molecular Biology(all)
Physics and Astronomy(all)

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Neri, T., Hiriart, E., van Vliet, P. P., Faure, E., Norris, R. A., Farhat, B., Jagla, B., Lefrancois, J., Sugi, Y., Moore-Morris, T., Zaffran, S., Faustino, R. S., Zambon, A. C., Desvignes, J. P., Salgado, D., Levine, R. A., de la Pompa, J. L., Terzic, A., Evans, S. M., ... Pucéat, M. (2019). Human pre-valvular endocardial cells derived from pluripotent stem cells recapitulate cardiac pathophysiological valvulogenesis. Nature communications, 10(1), Article 1929. https://doi.org/10.1038/s41467-019-09459-5

Neri, T, Hiriart, E, van Vliet, PP, Faure, E, Norris, RA, Farhat, B, Jagla, B, Lefrancois, J, Sugi, Y, Moore-Morris, T, Zaffran, S, Faustino, RS, Zambon, AC, Desvignes, JP, Salgado, D, Levine, RA, de la Pompa, JL, Terzic, A, Evans, SM, Markwald, R & Pucéat, M 2019, 'Human pre-valvular endocardial cells derived from pluripotent stem cells recapitulate cardiac pathophysiological valvulogenesis', Nature communications, vol. 10, no. 1, 1929. https://doi.org/10.1038/s41467-019-09459-5

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abstract = "Genetically modified mice have advanced our understanding of valve development and disease. Yet, human pathophysiological valvulogenesis remains poorly understood. Here we report that, by combining single cell sequencing and in vivo approaches, a population of human pre-valvular endocardial cells (HPVCs) can be derived from pluripotent stem cells. HPVCs express gene patterns conforming to the E9.0 mouse atrio-ventricular canal (AVC) endocardium signature. HPVCs treated with BMP2, cultured on mouse AVC cushions, or transplanted into the AVC of embryonic mouse hearts, undergo endothelial-to-mesenchymal transition and express markers of valve interstitial cells of different valvular layers, demonstrating cell specificity. Extending this model to patient-specific induced pluripotent stem cells recapitulates features of mitral valve prolapse and identified dysregulation of the SHH pathway. Concurrently increased ECM secretion can be rescued by SHH inhibition, thus providing a putative therapeutic target. In summary, we report a human cell model of valvulogenesis that faithfully recapitulates valve disease in a dish.",

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AU - Neri, Tui

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AU - van Vliet, Patrick P.

AU - Faure, Emilie

AU - Norris, Russell A.

AU - Farhat, Batoul

AU - Jagla, Bernd

AU - Lefrancois, Julie

AU - Sugi, Yukiko

AU - Moore-Morris, Thomas

AU - Zaffran, Stéphane

AU - Faustino, Randolph S.

AU - Zambon, Alexander C.

AU - Desvignes, Jean Pierre

AU - Salgado, David

AU - Levine, Robert A.

AU - de la Pompa, Jose Luis

AU - Terzic, André

AU - Evans, Sylvia M.

AU - Markwald, Roger

AU - Pucéat, Michel

PY - 2019/12/1

Y1 - 2019/12/1

N2 - Genetically modified mice have advanced our understanding of valve development and disease. Yet, human pathophysiological valvulogenesis remains poorly understood. Here we report that, by combining single cell sequencing and in vivo approaches, a population of human pre-valvular endocardial cells (HPVCs) can be derived from pluripotent stem cells. HPVCs express gene patterns conforming to the E9.0 mouse atrio-ventricular canal (AVC) endocardium signature. HPVCs treated with BMP2, cultured on mouse AVC cushions, or transplanted into the AVC of embryonic mouse hearts, undergo endothelial-to-mesenchymal transition and express markers of valve interstitial cells of different valvular layers, demonstrating cell specificity. Extending this model to patient-specific induced pluripotent stem cells recapitulates features of mitral valve prolapse and identified dysregulation of the SHH pathway. Concurrently increased ECM secretion can be rescued by SHH inhibition, thus providing a putative therapeutic target. In summary, we report a human cell model of valvulogenesis that faithfully recapitulates valve disease in a dish.

AB - Genetically modified mice have advanced our understanding of valve development and disease. Yet, human pathophysiological valvulogenesis remains poorly understood. Here we report that, by combining single cell sequencing and in vivo approaches, a population of human pre-valvular endocardial cells (HPVCs) can be derived from pluripotent stem cells. HPVCs express gene patterns conforming to the E9.0 mouse atrio-ventricular canal (AVC) endocardium signature. HPVCs treated with BMP2, cultured on mouse AVC cushions, or transplanted into the AVC of embryonic mouse hearts, undergo endothelial-to-mesenchymal transition and express markers of valve interstitial cells of different valvular layers, demonstrating cell specificity. Extending this model to patient-specific induced pluripotent stem cells recapitulates features of mitral valve prolapse and identified dysregulation of the SHH pathway. Concurrently increased ECM secretion can be rescued by SHH inhibition, thus providing a putative therapeutic target. In summary, we report a human cell model of valvulogenesis that faithfully recapitulates valve disease in a dish.

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Human pre-valvular endocardial cells derived from pluripotent stem cells recapitulate cardiac pathophysiological valvulogenesis

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