Homogentisic acid interference in routine urine creatinine determination

Perry R. Loken, Mark J. Magera, Wendy Introne, Silvia Tortorelli, Dimitar Gavrilov, Devin Oglesbee, Piero Rinaldo, Dietrich Matern, Kimiyo Raymond

Research output: Contribution to journalArticlepeer-review

11 Scopus citations


We report the artifactual elevation of homogentisic acid (HGA) in urine from alkaptonuric patients after replacing the creatinine method (Jaffe reaction) in our laboratory with an automated enzymatic method. Samples with elevated HGA by GC-MS had lower creatinine values as determined by the enzymatic method than by the Jaffe reaction. The low creatinine values were due to interference by HGA in the enzymatic method. The enzymatic method is unsuitable for creatinine determination in urine of patients with alkaptonuria.

Original languageEnglish (US)
Pages (from-to)103-104
Number of pages2
JournalMolecular Genetics and Metabolism
Issue number1
StatePublished - May 1 2010


  • Alkaptonuria
  • Creatinine
  • Homogentisate 1,2-dioxygenase
  • Homogentisic acid
  • Interference
  • Jaffe reaction

ASJC Scopus subject areas

  • Endocrinology, Diabetes and Metabolism
  • Biochemistry
  • Molecular Biology
  • Genetics
  • Endocrinology


Dive into the research topics of 'Homogentisic acid interference in routine urine creatinine determination'. Together they form a unique fingerprint.

Cite this