Homogentisic acid interference in routine urine creatinine determination

Perry R. Loken; Mark J. Magera; Wendy Introne; Silvia Tortorelli; Dimitar Gavrilov; Devin Oglesbee; Piero Rinaldo; Dietrich Matern; Kimiyo Raymond

doi:10.1016/j.ymgme.2010.01.006

Homogentisic acid interference in routine urine creatinine determination

Perry R. Loken, Mark J. Magera, Wendy Introne, Silvia Tortorelli, Dimitar Gavrilov, Devin Oglesbee, Piero Rinaldo, Dietrich Matern, Kimiyo Raymond

Laboratory Medicine and Pathology

Research output: Contribution to journal › Article › peer-review

11 Scopus citations

Abstract

We report the artifactual elevation of homogentisic acid (HGA) in urine from alkaptonuric patients after replacing the creatinine method (Jaffe reaction) in our laboratory with an automated enzymatic method. Samples with elevated HGA by GC-MS had lower creatinine values as determined by the enzymatic method than by the Jaffe reaction. The low creatinine values were due to interference by HGA in the enzymatic method. The enzymatic method is unsuitable for creatinine determination in urine of patients with alkaptonuria.

Original language	English (US)
Pages (from-to)	103-104
Number of pages	2
Journal	Molecular Genetics and Metabolism
Volume	100
Issue number	1
DOIs	https://doi.org/10.1016/j.ymgme.2010.01.006
State	Published - May 1 2010

Keywords

Alkaptonuria
Creatinine
Homogentisate 1,2-dioxygenase
Homogentisic acid
Interference
Jaffe reaction

ASJC Scopus subject areas

Endocrinology, Diabetes and Metabolism
Biochemistry
Molecular Biology
Genetics
Endocrinology

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10.1016/j.ymgme.2010.01.006

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title = "Homogentisic acid interference in routine urine creatinine determination",

abstract = "We report the artifactual elevation of homogentisic acid (HGA) in urine from alkaptonuric patients after replacing the creatinine method (Jaffe reaction) in our laboratory with an automated enzymatic method. Samples with elevated HGA by GC-MS had lower creatinine values as determined by the enzymatic method than by the Jaffe reaction. The low creatinine values were due to interference by HGA in the enzymatic method. The enzymatic method is unsuitable for creatinine determination in urine of patients with alkaptonuria.",

keywords = "Alkaptonuria, Creatinine, Homogentisate 1,2-dioxygenase, Homogentisic acid, Interference, Jaffe reaction",

author = "Loken, {Perry R.} and Magera, {Mark J.} and Wendy Introne and Silvia Tortorelli and Dimitar Gavrilov and Devin Oglesbee and Piero Rinaldo and Dietrich Matern and Kimiyo Raymond",

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doi = "10.1016/j.ymgme.2010.01.006",

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T1 - Homogentisic acid interference in routine urine creatinine determination

AU - Loken, Perry R.

AU - Magera, Mark J.

AU - Introne, Wendy

AU - Tortorelli, Silvia

AU - Gavrilov, Dimitar

AU - Oglesbee, Devin

AU - Rinaldo, Piero

AU - Matern, Dietrich

AU - Raymond, Kimiyo

PY - 2010/5/1

Y1 - 2010/5/1

N2 - We report the artifactual elevation of homogentisic acid (HGA) in urine from alkaptonuric patients after replacing the creatinine method (Jaffe reaction) in our laboratory with an automated enzymatic method. Samples with elevated HGA by GC-MS had lower creatinine values as determined by the enzymatic method than by the Jaffe reaction. The low creatinine values were due to interference by HGA in the enzymatic method. The enzymatic method is unsuitable for creatinine determination in urine of patients with alkaptonuria.

AB - We report the artifactual elevation of homogentisic acid (HGA) in urine from alkaptonuric patients after replacing the creatinine method (Jaffe reaction) in our laboratory with an automated enzymatic method. Samples with elevated HGA by GC-MS had lower creatinine values as determined by the enzymatic method than by the Jaffe reaction. The low creatinine values were due to interference by HGA in the enzymatic method. The enzymatic method is unsuitable for creatinine determination in urine of patients with alkaptonuria.

KW - Alkaptonuria

KW - Creatinine

KW - Homogentisate 1,2-dioxygenase

KW - Homogentisic acid

KW - Interference

KW - Jaffe reaction

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JO - Molecular Genetics and Metabolism

JF - Molecular Genetics and Metabolism

IS - 1

ER -

Homogentisic acid interference in routine urine creatinine determination

Abstract

Keywords

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