High-throughput analysis of tissue microarrays using automated desorption electrospray ionization mass spectrometry

Nicolás M. Morato, Hannah Marie Brown, Diogo Garcia, Erik H. Middlebrooks, Mark Jentoft, Kaisorn Chaichana, Alfredo Quiñones-Hinojosa, R. Graham Cooks

Research output: Contribution to journalArticlepeer-review


Tissue microarrays (TMAs) are commonly used for the rapid analysis of large numbers of tissue samples, often in morphological assessments but increasingly in spectroscopic analysis, where specific molecular markers are targeted via immunostaining. Here we report the use of an automated high-throughput system based on desorption electrospray ionization (DESI) mass spectrometry (MS) for the rapid generation and online analysis of high-density (6144 samples/array) TMAs, at rates better than 1 sample/second. Direct open-air analysis of tissue samples (hundreds of nanograms) not subjected to prior preparation, plus the ability to provide molecular characterization by tandem mass spectrometry (MS/MS), make this experiment versatile and applicable to both targeted and untargeted analysis in a label-free manner. These capabilities are demonstrated in a proof-of-concept study of frozen brain tissue biopsies where we showcase (i) a targeted MS/MS application aimed at identification of isocitrate dehydrogenase mutation in glioma samples and (ii) an untargeted MS tissue type classification using lipid profiles and correlation with tumor cell percentage estimates from histopathology. The small sample sizes and large sample numbers accessible with this methodology make for a powerful analytical system that facilitates the identification of molecular markers for later use in intraoperative applications to guide precision surgeries and ultimately improve patient outcomes.

Original languageEnglish (US)
Article number18851
JournalScientific reports
Issue number1
StatePublished - Dec 2022

ASJC Scopus subject areas

  • General


Dive into the research topics of 'High-throughput analysis of tissue microarrays using automated desorption electrospray ionization mass spectrometry'. Together they form a unique fingerprint.

Cite this