Glucocorticoid regulation of rRNA synthesis

Pramod B. Mahajan; E. Aubrey Thompson

doi:10.1007/BF00229820

Glucocorticoid regulation of rRNA synthesis

Pramod B. Mahajan, E. Aubrey Thompson

Cancer Biology

Research output: Contribution to journal › Article › peer-review

9 Scopus citations

Abstract

Glucocorticoids inhibit transcription of the genes encoding rRNA (rDNA) in P1798 lymphoma cells. This is due to a decrease in the amount or activity of a transcription factor, called TFIC. TFIC has been purified to apparent homogeneity and the properties of this protein have been investigated in detail. TFIC is tightly associated with RNA polymerase I. The data indicate that TFIC is a bonafide initiation factor that is required for formation of the first phosphodiester bond of nascent pre-rRNA. Extracts from dexamethasone-treated cells are devoid of this factor and cannot form initiated complexes in vitro.

Original language	English (US)
Pages (from-to)	195-200
Number of pages	6
Journal	Molecular and Cellular Biochemistry
Volume	104
Issue number	1-2
DOIs	https://doi.org/10.1007/BF00229820
State	Published - May 1 1991

Keywords

dexamethasone
glucocorticoids
rRNA synthesis
transcription factors
transcription in vitro

ASJC Scopus subject areas

Molecular Biology
Clinical Biochemistry
Cell Biology

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10.1007/BF00229820

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AB - Glucocorticoids inhibit transcription of the genes encoding rRNA (rDNA) in P1798 lymphoma cells. This is due to a decrease in the amount or activity of a transcription factor, called TFIC. TFIC has been purified to apparent homogeneity and the properties of this protein have been investigated in detail. TFIC is tightly associated with RNA polymerase I. The data indicate that TFIC is a bonafide initiation factor that is required for formation of the first phosphodiester bond of nascent pre-rRNA. Extracts from dexamethasone-treated cells are devoid of this factor and cannot form initiated complexes in vitro.

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KW - glucocorticoids

KW - rRNA synthesis

KW - transcription factors

KW - transcription in vitro

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Glucocorticoid regulation of rRNA synthesis

Abstract

Keywords

ASJC Scopus subject areas

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