Foxa1 defines cancer cell specificity

Gaihua Zhang, Yongbing Zhao, Yi Liu, Li Pin Kao, Xiao Wang, Benjamin Skerry, Zhaoyu Li

Research output: Contribution to journalArticlepeer-review

23 Scopus citations


A transcription factor functions differentially and/or identically in multiple cell types. However, the mechanism for cell-specific regulation of a transcription factor remains to be elucidated. We address how a single transcription factor, forkhead box protein A1 (FOXA1), forms cell-specific genomic signatures and differentially regulates gene expression in four human cancer cell lines (HepG2, LNCaP, MCF7, and T47D). FOXA1 is a pioneer transcription factor in organogenesis and cancer progression. Genomewide mapping of FOXA1 by chromatin immunoprecipitation sequencing annotates that target genes associated with FOXA1 binding are mostly common to these cancer cells. However, most of the functional FOXA1 target genes are specific to each cancer cell type. Further investigations using CRISPR-Cas9 genome editing technology indicate that cell-specific FOXA1 regulation is attributable to unique FOXA1 binding, genetic variations, and/or potential epigenetic regulation. Thus, FOXA1 controls the specificity of cancer cell types. We raise a "flower-blooming" hypothesis for cell-specific transcriptional regulation based on these observations.

Original languageEnglish (US)
Article numbere1501473
JournalScience Advances
Issue number3
StatePublished - Mar 2016

ASJC Scopus subject areas

  • General


Dive into the research topics of 'Foxa1 defines cancer cell specificity'. Together they form a unique fingerprint.

Cite this