Feasibility of medical image-based bio 3D printing of variable tissue compositions

Rohit Shinde, Dietrich James P. Nigh, Sofia M. Miguez, Sylvia S. Rhodes, Jayaram K. Udupa, Snehal Shetye, Abhishek Chandra, Robert Pignolo, Chamith S. Rajapakse

Research output: Chapter in Book/Report/Conference proceedingConference contribution


This study aims to display the ability and efficacy of 3D printing image-based, implantable biological scaffolds with varying properties. In this study, scaffolds were printed using various ratios of hydroxyapatite (HA) to polycaprolactone (PCL) to display a spectrum of properties suitable for musculoskeletal scaffolds. As an initial application of this method, scaffolds were generated from a series of one hundred DICOM images for a 60-year-old, female proximal femur. Additional structures, including a printed box and a circular lattice were generated. These models were printed at HA to PCL ratios (m/m) of 1:9, 2:8, 3:7, 4:6, 5:5, 6:4, 7:3, 8:2, and 9:1. Postprinting analysis of the ratios was performed with scanning electron microscopy to observe the prints' microstructure. Post printing analysis also included a compression test to observe biomechanical properties and a cell culture on the prints to observe cellular viability and adhesion. Ratios showed vast microstructural differences. It was also found that the 6:4 sample had the most similar surface level microstructure to that of human trabecular bone. The compression test revealed a positive correlation (R2 = 0.92) between HA concentration (%) and stiffness (N/mm). Cellular viability and adhesion were confirmed for 10 days after initial seeding cells.

Original languageEnglish (US)
Title of host publicationMedical Imaging 2019
Subtitle of host publicationImaging Informatics for Healthcare, Research, and Applications
EditorsPeter R. Bak, Po-Hao Chen
ISBN (Electronic)9781510625556
StatePublished - Jan 1 2019
EventMedical Imaging 2019: Imaging Informatics for Healthcare, Research, and Applications - San Diego, United States
Duration: Feb 17 2019Feb 18 2019

Publication series

NameProgress in Biomedical Optics and Imaging - Proceedings of SPIE
ISSN (Print)1605-7422


ConferenceMedical Imaging 2019: Imaging Informatics for Healthcare, Research, and Applications
Country/TerritoryUnited States
CitySan Diego


  • 3D-printing
  • Biomechanical
  • Cellular viability
  • Hydroxyapatite
  • Image-based
  • Microstructure
  • Patient-specific
  • Polycaprolactone
  • Scaffold

ASJC Scopus subject areas

  • Electronic, Optical and Magnetic Materials
  • Atomic and Molecular Physics, and Optics
  • Biomaterials
  • Radiology Nuclear Medicine and imaging


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