TY - JOUR
T1 - Extra-cellular vesicles carry proteome of cancer hallmarks
AU - Carvalho, Ana Sofia
AU - Baeta, Henrique
AU - Silva, Bruno Costa
AU - Moraes, Maria Carolina Strano
AU - Bodo, Cristian
AU - Beck, Hans Christian
AU - Rodriguez, Manuel S.
AU - Saraswat, Mayank
AU - Pandey, Akhilesh
AU - Matthiesen, Rune
N1 - Funding Information:
R.M. is supported by Fundação para a Ciência e a Tecnologia (CEEC position, 2019-2025 investigator). This article is a result of the projects (iNOVA4Health - UID/Multi/04462/2013), supported by Lisboa Portugal Regional Operational Programme (Lisboa2020), under the PORTUGAL 2020 Partnership Agreement, through the European Regional Development Fund (ERDF). This work is also funded by FEDER funds through the COMPETE 2020 Programme and National Funds through FCT - Portuguese Foundation for Science and Technology under the projects number PTDC/BTM-TEC/30087/2017 and PTDC/BTM-TEC/30088/2017. This work was supported by the Wellcome Trust/DBT India Alliance Margdarshi Fellowship (grant number IA/M/15/1/502023) awarded to A. P. B.C.S, M.C.S.C. and C.B. are supported by the the Champalimaud Foundation and the EMBO Installation Grant 3921. The results shown here are in part based upon data generated by the TCGA Research Network: https://www.cancer.gov/tcga.
Publisher Copyright:
© 2020 Frontiers in Bioscience. All rights reserved.
PY - 2020/1/1
Y1 - 2020/1/1
N2 - Through lateral transfer, extra-cellular vesicles (EVs) transport their DNA, miRNA, mRNA and proteins such as enzymes mediating drug resistance, transporters as well as growth factors to neighboring cells. By virtue of this horizontal transfer, EVs potentially regulate cell growth, migration, angiogenesis and metastasis and increase tissue permeability in cancer. Furthermore, EVs regulate immune factors and allow the tumor cells to evade immune recognition and cell death. To explore if the proteomes of exosomes support functional transfer of cancer hallmarks, in this meta-analysis, we compared EVs and whole cell proteomes from the NCI-60 human tumor cell line panel. We observed a subgroup of proteins in each cancer hallmark signature as highly abundant and consistently expressed in EVs from all cell lines. Among these were oncoproteins frequently targeted in cancer therapies whose presence on EVs could potentially render therapies less effective by serving as decoys.
AB - Through lateral transfer, extra-cellular vesicles (EVs) transport their DNA, miRNA, mRNA and proteins such as enzymes mediating drug resistance, transporters as well as growth factors to neighboring cells. By virtue of this horizontal transfer, EVs potentially regulate cell growth, migration, angiogenesis and metastasis and increase tissue permeability in cancer. Furthermore, EVs regulate immune factors and allow the tumor cells to evade immune recognition and cell death. To explore if the proteomes of exosomes support functional transfer of cancer hallmarks, in this meta-analysis, we compared EVs and whole cell proteomes from the NCI-60 human tumor cell line panel. We observed a subgroup of proteins in each cancer hallmark signature as highly abundant and consistently expressed in EVs from all cell lines. Among these were oncoproteins frequently targeted in cancer therapies whose presence on EVs could potentially render therapies less effective by serving as decoys.
KW - Biomarkers
KW - Cancer exosomes
KW - Cancer extracellular microvesicles
KW - Drug resistance
KW - Proteomics
KW - Review
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UR - http://www.scopus.com/inward/citedby.url?scp=85072941462&partnerID=8YFLogxK
U2 - 10.2741/4811
DO - 10.2741/4811
M3 - Article
C2 - 31585894
AN - SCOPUS:85072941462
SN - 2768-6701
VL - 25
SP - 398
EP - 436
JO - Frontiers in Bioscience - Landmark
JF - Frontiers in Bioscience - Landmark
IS - 3
ER -