TY - JOUR
T1 - Evaluation of a genus-and group-specific rapid PCR assay panel on synovial fluid for diagnosis of prosthetic knee infection
AU - Melendez, Dante P.
AU - Greenwood-Quaintance, Kerryl E.
AU - Berbari, Elie F.
AU - Osmon, Douglas R.
AU - Mandrekar, Jayawant N.
AU - Hanssen, Arlen D.
AU - Patel, Robin
N1 - Funding Information:
We thank Melissa J. Karau and Suzannah M. Schmidt-Malan for collection and storage of synovial fluid specimens. We thank the outstanding technologists in the Mayo Clinic clinical bacteriology and initial processing laboratories for performing the cultures. HHS| National Institutes of Health (NIH) provided funding to Robin Patel under grant numbers R01 AR056647 and R01 AI91594. HHS | NIH | National Center for Advancing Translational Sciences (NCATS) provided funding to Dante P Melendez under grant number UL1 TR000135. The funders had no role in study design, data collection and interpretation, or the decision to submit the work for publication.
Publisher Copyright:
© 2015, American Society for Microbiology. All Rights Reserved.
PY - 2016/1
Y1 - 2016/1
N2 - We evaluated a genus-and group-specific PCR assay panel using 284 prosthetic knee synovial fluid samples collected from patients presenting to our institution with implant failure. Using the Musculoskeletal Infection Society diagnostic criteria, 88 and 196 samples were classified as showing prosthetic joint infection (PJI) and aseptic failure (AF), respectively. Sensitivities of the synovial fluid PCR panel and culture were 55.6% and 76.1% (P<0.001), respectively, and specificities were 91.8% and 97.4% (P≤0.016), respectively. Among the 70 subjects who had received antibiotics within the month preceding synovial fluid aspiration (48 of whom had PJI), PCR panel and synovial fluid culture sensitivities were 64.5% and 85.4%, respectively (P<0.0001). In this group, the PCR panel detected Staphylococcus aureus in two culture-negative PJI cases. Overall, the evaluated molecular diagnostic tool had low sensitivity when applied to synovial fluid.
AB - We evaluated a genus-and group-specific PCR assay panel using 284 prosthetic knee synovial fluid samples collected from patients presenting to our institution with implant failure. Using the Musculoskeletal Infection Society diagnostic criteria, 88 and 196 samples were classified as showing prosthetic joint infection (PJI) and aseptic failure (AF), respectively. Sensitivities of the synovial fluid PCR panel and culture were 55.6% and 76.1% (P<0.001), respectively, and specificities were 91.8% and 97.4% (P≤0.016), respectively. Among the 70 subjects who had received antibiotics within the month preceding synovial fluid aspiration (48 of whom had PJI), PCR panel and synovial fluid culture sensitivities were 64.5% and 85.4%, respectively (P<0.0001). In this group, the PCR panel detected Staphylococcus aureus in two culture-negative PJI cases. Overall, the evaluated molecular diagnostic tool had low sensitivity when applied to synovial fluid.
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U2 - 10.1128/JCM.02302-15
DO - 10.1128/JCM.02302-15
M3 - Article
C2 - 26537446
AN - SCOPUS:84954316731
SN - 0095-1137
VL - 54
SP - 120
EP - 126
JO - Journal of clinical microbiology
JF - Journal of clinical microbiology
IS - 1
ER -