Establishment and Characterization of a New Human Intrahepatic Cholangiocarcinoma Cell Line LIV27

Xiwei Ding; Kais Zakharia; Catherine D. Moser; Nellie A. Campbell; Chunling Hu; Nataliya Razumilava; Roongruedee Chaiteerakij; Hassan M. Shaleh; Patricia T. Greipp; Rondell P. Graham; Xiaoping Zou; Vishal S. Chandan; Lewis R. Roberts

doi:10.3390/cancers14205080

Establishment and Characterization of a New Human Intrahepatic Cholangiocarcinoma Cell Line LIV27

Xiwei Ding, Kais Zakharia, Catherine D. Moser, Nellie A. Campbell, Chunling Hu, Nataliya Razumilava, Roongruedee Chaiteerakij, Hassan M. Shaleh, Patricia T. Greipp, Rondell P. Graham, Xiaoping Zou, Vishal S. Chandan, Lewis R. Roberts

Research output: Contribution to journal › Article › peer-review

Abstract

Cholangiocarcinoma (CCA) is a highly lethal cancer arising from the biliary tract epithelium. The cancer biology of this neoplasm is not well understood. To date, only a few CCA cell lines have been reported, which were mostly developed from Asian patients. In this study, we report and characterize a new intrahepatic CCA cell line, LIV27, derived from a surgically resected tumor in a 67-year-old Caucasian woman with primary sclerosing cholangitis (PSC). LIV27 cells grow well in collagen-coated flasks or plates with a doubling time of 57.8 h at passage 14. LIV27 cells have high tumorigenicity in nude mice and stain positive for CK7 and CK19, markers that differentiate CCA from hepatocellular carcinoma. Karyotype analysis showed that LIV27 is aneuploid. We established a single-locus short tandem repeat profile for the LIV27 cell line. This newly established cell line will be a useful model for studying the molecular pathogenesis of, and developing novel therapies for, cholangiocarcinoma.

Original language	English (US)
Article number	5080
Journal	Cancers
Volume	14
Issue number	20
DOIs	https://doi.org/10.3390/cancers14205080
State	Published - Oct 2022

Keywords

LIV27
cell line
cholangiocarcinoma
primary sclerosing cholangitis
short tandem repeat

ASJC Scopus subject areas

Oncology
Cancer Research

Access to Document

10.3390/cancers14205080

Cite this

@article{b169651ac8ca40c3937825dbaa17eef9,

title = "Establishment and Characterization of a New Human Intrahepatic Cholangiocarcinoma Cell Line LIV27",

abstract = "Cholangiocarcinoma (CCA) is a highly lethal cancer arising from the biliary tract epithelium. The cancer biology of this neoplasm is not well understood. To date, only a few CCA cell lines have been reported, which were mostly developed from Asian patients. In this study, we report and characterize a new intrahepatic CCA cell line, LIV27, derived from a surgically resected tumor in a 67-year-old Caucasian woman with primary sclerosing cholangitis (PSC). LIV27 cells grow well in collagen-coated flasks or plates with a doubling time of 57.8 h at passage 14. LIV27 cells have high tumorigenicity in nude mice and stain positive for CK7 and CK19, markers that differentiate CCA from hepatocellular carcinoma. Karyotype analysis showed that LIV27 is aneuploid. We established a single-locus short tandem repeat profile for the LIV27 cell line. This newly established cell line will be a useful model for studying the molecular pathogenesis of, and developing novel therapies for, cholangiocarcinoma.",

keywords = "LIV27, cell line, cholangiocarcinoma, primary sclerosing cholangitis, short tandem repeat",

author = "Xiwei Ding and Kais Zakharia and Moser, {Catherine D.} and Campbell, {Nellie A.} and Chunling Hu and Nataliya Razumilava and Roongruedee Chaiteerakij and Shaleh, {Hassan M.} and Greipp, {Patricia T.} and Graham, {Rondell P.} and Xiaoping Zou and Chandan, {Vishal S.} and Roberts, {Lewis R.}",

note = "Funding Information: This work was supported by the National Institutes of Health (CA165076), the Mayo Clinic Center for Cell Signaling in Gastroenterology (P30DK084567), the Mayo Clinic Cancer Center (CA15083), the Mayo Clinic SPORE in Hepatobiliary Cancer (P50CA210964), and the Cholangiocarcinoma Foundation (to L.R.R.). Publisher Copyright: {\textcopyright} 2022 by the authors.",

year = "2022",

month = oct,

doi = "10.3390/cancers14205080",

language = "English (US)",

volume = "14",

journal = "Cancers",

issn = "2072-6694",

publisher = "Multidisciplinary Digital Publishing Institute (MDPI)",

number = "20",

}

TY - JOUR

T1 - Establishment and Characterization of a New Human Intrahepatic Cholangiocarcinoma Cell Line LIV27

AU - Ding, Xiwei

AU - Zakharia, Kais

AU - Moser, Catherine D.

AU - Campbell, Nellie A.

AU - Hu, Chunling

AU - Razumilava, Nataliya

AU - Chaiteerakij, Roongruedee

AU - Shaleh, Hassan M.

AU - Greipp, Patricia T.

AU - Graham, Rondell P.

AU - Zou, Xiaoping

AU - Chandan, Vishal S.

AU - Roberts, Lewis R.

N1 - Funding Information: This work was supported by the National Institutes of Health (CA165076), the Mayo Clinic Center for Cell Signaling in Gastroenterology (P30DK084567), the Mayo Clinic Cancer Center (CA15083), the Mayo Clinic SPORE in Hepatobiliary Cancer (P50CA210964), and the Cholangiocarcinoma Foundation (to L.R.R.). Publisher Copyright: © 2022 by the authors.

PY - 2022/10

Y1 - 2022/10

N2 - Cholangiocarcinoma (CCA) is a highly lethal cancer arising from the biliary tract epithelium. The cancer biology of this neoplasm is not well understood. To date, only a few CCA cell lines have been reported, which were mostly developed from Asian patients. In this study, we report and characterize a new intrahepatic CCA cell line, LIV27, derived from a surgically resected tumor in a 67-year-old Caucasian woman with primary sclerosing cholangitis (PSC). LIV27 cells grow well in collagen-coated flasks or plates with a doubling time of 57.8 h at passage 14. LIV27 cells have high tumorigenicity in nude mice and stain positive for CK7 and CK19, markers that differentiate CCA from hepatocellular carcinoma. Karyotype analysis showed that LIV27 is aneuploid. We established a single-locus short tandem repeat profile for the LIV27 cell line. This newly established cell line will be a useful model for studying the molecular pathogenesis of, and developing novel therapies for, cholangiocarcinoma.

AB - Cholangiocarcinoma (CCA) is a highly lethal cancer arising from the biliary tract epithelium. The cancer biology of this neoplasm is not well understood. To date, only a few CCA cell lines have been reported, which were mostly developed from Asian patients. In this study, we report and characterize a new intrahepatic CCA cell line, LIV27, derived from a surgically resected tumor in a 67-year-old Caucasian woman with primary sclerosing cholangitis (PSC). LIV27 cells grow well in collagen-coated flasks or plates with a doubling time of 57.8 h at passage 14. LIV27 cells have high tumorigenicity in nude mice and stain positive for CK7 and CK19, markers that differentiate CCA from hepatocellular carcinoma. Karyotype analysis showed that LIV27 is aneuploid. We established a single-locus short tandem repeat profile for the LIV27 cell line. This newly established cell line will be a useful model for studying the molecular pathogenesis of, and developing novel therapies for, cholangiocarcinoma.

KW - LIV27

KW - cell line

KW - cholangiocarcinoma

KW - primary sclerosing cholangitis

KW - short tandem repeat

UR - http://www.scopus.com/inward/record.url?scp=85140595915&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=85140595915&partnerID=8YFLogxK

U2 - 10.3390/cancers14205080

DO - 10.3390/cancers14205080

M3 - Article

AN - SCOPUS:85140595915

SN - 2072-6694

VL - 14

JO - Cancers

JF - Cancers

IS - 20

M1 - 5080

ER -

Establishment and Characterization of a New Human Intrahepatic Cholangiocarcinoma Cell Line LIV27

Abstract

Keywords

ASJC Scopus subject areas

Access to Document

Other files and links

Fingerprint

Cite this