Eosinophils promote effector functions of lung group 2 innate lymphoid cells in allergic airway inflammation in mice

William E. LeSuer, Melanie Kienzl, Sergei I. Ochkur, Rudolf Schicho, Alfred D. Doyle, Benjamin L. Wright, Matthew A. Rank, Alexander S. Krupnick, Hirohito Kita, Elizabeth A. Jacobsen

Research output: Contribution to journalArticlepeer-review


Background: Group 2 innate lymphoid cells (ILC2s) are critical mediators of type 2 respiratory inflammation, releasing IL-5 and IL-13 and promoting the pulmonary eosinophilia associated with allergen provocation. Although ILC2s have been shown to promote eosinophil activities, the role of eosinophils in group 2 innate lymphoid cell (ILC2) responses is less well defined. Objective: We sought to investigate the role of eosinophils in activation of ILC2s in models of allergic asthma and in vitro. Methods: Inducible eosinophil-deficient mice were exposed to allergic respiratory inflammation models of asthma, such as ovalbumin or house dust mite challenge, or to innate models of type 2 airway inflammation, such as inhalation of IL-33. Eosinophil-specific IL-4/13–deficient mice were used to address the specific roles for eosinophil-derived cytokines. Direct cell interactions between ILC2s and eosinophils were assessed by in vitro culture experiments. Results: Targeted depletion of eosinophils resulted in significant reductions of total and IL-5+ and IL-13+ lung ILC2s in all models of respiratory inflammation. This correlated with reductions in IL-13 levels and mucus in the airway. Eosinophil-derived IL-4/13 was necessary for both eosinophil and ILC2 accumulation in lung in allergen models. In vitro, eosinophils released soluble mediators that induced ILC2 proliferation and G protein–coupled receptor–dependent chemotaxis of ILC2s. Coculture of ILC2s and IL-33–activated eosinophils resulted in transcriptome changes in both ILC2s and eosinophils, suggesting potential novel reciprocal interactions. Conclusion: These studies demonstrate that eosinophils play a reciprocal role in ILC2 effector functions as part of both adaptive and innate type 2 pulmonary inflammatory events.

Original languageEnglish (US)
Pages (from-to)469-485.e10
JournalJournal of Allergy and Clinical Immunology
Issue number2
StatePublished - Aug 2023


  • Eosinophil
  • IL-13
  • IL-33
  • IL-4
  • asthma
  • eosinophil deficient
  • group 2 innate lymphoid cell
  • inflammation
  • lung

ASJC Scopus subject areas

  • Immunology and Allergy
  • Immunology


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