Enteroendocrine cells and 5-HT availability are altered in mucosa of guinea pigs with TNBS ileitis

Jennifer R. O'Hara, Winnie Ho, David R. Linden, Gary M. Mawe, Keith A. Sharkey

Research output: Contribution to journalArticlepeer-review

105 Scopus citations


Enteroendocrine cells act as sensory transducers, releasing 5-HT and numerous peptides that are involved in regulating motility, secretion, and gut sensation. The action of mucosal 5-HT is terminated by a 5-HT reuptake transporter (SERT). In this study, we examined the hypothesis that ileitis leads to changes in enteroendocrine cell populations and mucosal 5-HT availability. Ileitis was induced in guinea pigs by intraluminal injection of 2,4,6-trinitrobenzenesulfonic acid and experiments were conducted 3, 7, and 14 days after treatment. The number of somatostatin, neurotensin, and 5-HT-immunoreactive cells increased at 3 and 7 days of ileitis, respectively, whereas no significant changes in the numbers of cholecystokinin, glucagon-like peptide-2, glucose-dependent insulinotropic peptide, and peptide YY-immunoreactive cells were observed. Chemical stimulation of the inflamed mucosa with sodium deoxycholic acid significantly increased 5-HT release compared with basal release. Mechanical stimulation of the mucosa potentiated the effect of the chemical stimuli at day 7. Epithelial SERT immunoreactivity was significantly reduced during the time course of inflammation. Thus changes in enteroendocrine cell populations and 5-HT availability could contribute to the altered motility and secretion associated with intestinal inflammation by disrupting mucosal signaling to enteric nerves involved in peristaltic and secretory reflexes.

Original languageEnglish (US)
Pages (from-to)G998-G1007
JournalAmerican Journal of Physiology - Gastrointestinal and Liver Physiology
Issue number5 50-5
StatePublished - Nov 2004


  • Inflammatory bowel disease
  • Motility
  • Neurotensin
  • Secretion
  • Sensory transduction
  • Somatostatin

ASJC Scopus subject areas

  • Physiology
  • Hepatology
  • Gastroenterology
  • Physiology (medical)


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