Diabetic neuropathy: An evaluation of the use of quercetin in the cecum of rats

Paulo Emilio Botura Ferreira, Cláudia Regina Pinheiro Lopes, Angela Maria Pereira Alves, Éder Paulo Belato Alves, David Robert Linden, Jacqueline Nelisis Zanoni, Nilza Cristina Buttow

Research output: Contribution to journalArticlepeer-review

30 Scopus citations


Aim: To investigate the effect of quercetin supplementation on the myenteric neurons and glia in the cecum of diabetic rats. Methods: Total preparations of the muscular tunic were prepared from the ceca of twenty-four rats divided into the following groups: control (C), control supplemented with quercetin (200 mg/kg quercetin body weight) (CQ), diabetic (D) and diabetic supplemented with quercetin (DQ). Immunohistochemical double staining technique was performed with HuC/D (general population)/nitric oxide synthase (nNOS), HuC-D/S-100 and VIP. Density analysis of the general neuronal population HuC/D-IR, the nNOS-IR (nitrergic subpopulation) and the enteric glial cells (S-100) was performed, and the morphometry and the reduction in varicosity population (VIP-IR) in these populations were analyzed. Results: Diabetes promoted a significant reduction (25%) in the neuronal density of the HuC/D-IR (general population) and the nNOS-IR (nitrergic subpopulation) compared with the C group. Diabetes also significantly increased the areas of neurons, glial cells and VIP-IR varicosities. Supplementation with quercetin in the DQ group prevented neuronal loss in the general population and increased its area (P < 0.001) and the area of nitrergic subpopulation (P < 0.001), when compared to C group. Quercetin induced a VIP-IR and glial cells areas (P < 0.001) in DQ group when compared to C, CQ and D groups. Conclusion: In diabetes, quercetin exhibited a neuroprotective effect by maintaining the density of the general neuronal population but did not affect the density of the nNOS subpopulation.

Original languageEnglish (US)
Pages (from-to)6416-6426
Number of pages11
JournalWorld journal of gastroenterology
Issue number38
StatePublished - Oct 14 2013


  • Diabetes
  • Enteric glia
  • Myenteric plexus
  • Neuronal nitric oxide synthase
  • Neuroprotection
  • Vasoactive intestinal polypeptide

ASJC Scopus subject areas

  • Gastroenterology


Dive into the research topics of 'Diabetic neuropathy: An evaluation of the use of quercetin in the cecum of rats'. Together they form a unique fingerprint.

Cite this