Cytogenetic analysis and construction of a BAC contig across a common neocentromeric region from 9p

D. L. Satinover; G. H. Vance; D. L. Van Dyke; S. Schwartz

doi:10.1007/s004120100143

Cytogenetic analysis and construction of a BAC contig across a common neocentromeric region from 9p

D. L. Satinover, G. H. Vance, D. L. Van Dyke, S. Schwartz

Laboratory Medicine and Pathology

Research output: Contribution to journal › Article › peer-review

26 Scopus citations

Abstract

Over 40 cases of neocentric marker chromosomes, without detectable α-satellite DNA, have been reported. Although these have originated from many different chromosomes, a few of these chromosomes have been involved in multiple cases of marker formation. In this study, two different markers originating from the short arm of chromosome 9 were analyzed, identifying a common neocentrometric region. A bacterial artificial chromosome (BAC) contig extending over more than 900 kb has been assembled across this neocentromeric region. Fluorescent in situ hybridization and immunofluorescence assays (CENP-C and CENP-E) have localized the neocentromere to a 500 kb region. Preliminary analysis of DNA sequences in this neocentromere revealed a highly AT-rich region, which also has an increase in the level of retroviral elements compared with the average levels in the genome.

Original language	English (US)
Pages (from-to)	275-283
Number of pages	9
Journal	Chromosoma
Volume	110
Issue number	4
DOIs	https://doi.org/10.1007/s004120100143
State	Published - 2001

ASJC Scopus subject areas

Genetics
Genetics(clinical)

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title = "Cytogenetic analysis and construction of a BAC contig across a common neocentromeric region from 9p",

abstract = "Over 40 cases of neocentric marker chromosomes, without detectable α-satellite DNA, have been reported. Although these have originated from many different chromosomes, a few of these chromosomes have been involved in multiple cases of marker formation. In this study, two different markers originating from the short arm of chromosome 9 were analyzed, identifying a common neocentrometric region. A bacterial artificial chromosome (BAC) contig extending over more than 900 kb has been assembled across this neocentromeric region. Fluorescent in situ hybridization and immunofluorescence assays (CENP-C and CENP-E) have localized the neocentromere to a 500 kb region. Preliminary analysis of DNA sequences in this neocentromere revealed a highly AT-rich region, which also has an increase in the level of retroviral elements compared with the average levels in the genome.",

author = "Satinover, {D. L.} and Vance, {G. H.} and {Van Dyke}, {D. L.} and S. Schwartz",

note = "Funding Information: Acknowledgements The authors wish to acknowledge Michele Eichenmiller for her technical assistance. We wish to thank Dr. John Harrington at Athersys for the antibodies for CENP-C and CENP-E. We also wish to thank Dr. Evan Eichler for his thoughtful input. This work was supported in part by NIH grant HD07518-03 and by a grant from the Wilson Foundation to the Center for Human Genetics at University Hospitals of Cleveland.",

year = "2001",

doi = "10.1007/s004120100143",

language = "English (US)",

volume = "110",

pages = "275--283",

journal = "Chromosoma",

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T1 - Cytogenetic analysis and construction of a BAC contig across a common neocentromeric region from 9p

AU - Satinover, D. L.

AU - Vance, G. H.

AU - Van Dyke, D. L.

AU - Schwartz, S.

N1 - Funding Information: Acknowledgements The authors wish to acknowledge Michele Eichenmiller for her technical assistance. We wish to thank Dr. John Harrington at Athersys for the antibodies for CENP-C and CENP-E. We also wish to thank Dr. Evan Eichler for his thoughtful input. This work was supported in part by NIH grant HD07518-03 and by a grant from the Wilson Foundation to the Center for Human Genetics at University Hospitals of Cleveland.

PY - 2001

Y1 - 2001

N2 - Over 40 cases of neocentric marker chromosomes, without detectable α-satellite DNA, have been reported. Although these have originated from many different chromosomes, a few of these chromosomes have been involved in multiple cases of marker formation. In this study, two different markers originating from the short arm of chromosome 9 were analyzed, identifying a common neocentrometric region. A bacterial artificial chromosome (BAC) contig extending over more than 900 kb has been assembled across this neocentromeric region. Fluorescent in situ hybridization and immunofluorescence assays (CENP-C and CENP-E) have localized the neocentromere to a 500 kb region. Preliminary analysis of DNA sequences in this neocentromere revealed a highly AT-rich region, which also has an increase in the level of retroviral elements compared with the average levels in the genome.

AB - Over 40 cases of neocentric marker chromosomes, without detectable α-satellite DNA, have been reported. Although these have originated from many different chromosomes, a few of these chromosomes have been involved in multiple cases of marker formation. In this study, two different markers originating from the short arm of chromosome 9 were analyzed, identifying a common neocentrometric region. A bacterial artificial chromosome (BAC) contig extending over more than 900 kb has been assembled across this neocentromeric region. Fluorescent in situ hybridization and immunofluorescence assays (CENP-C and CENP-E) have localized the neocentromere to a 500 kb region. Preliminary analysis of DNA sequences in this neocentromere revealed a highly AT-rich region, which also has an increase in the level of retroviral elements compared with the average levels in the genome.

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JO - Chromosoma

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IS - 4

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Cytogenetic analysis and construction of a BAC contig across a common neocentromeric region from 9p

Abstract

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