Cryptosporidium parvum induces B7-H1 expression in cholangiocytes by down-regulating microRNA-513

Ai Yu Gong, Rui Zhou, Guoku Hu, Jun Liu, Danuta Sosnowska, Kristen M. Drescher, Haidong Dong, Xian Ming Chen

Research output: Contribution to journalArticlepeer-review

50 Scopus citations


Expression of B7 costimulatory molecules represents an important compartment of immune response of epithelial cells after microbial infection. We report here that the protozoan parasite Cryptosporidium parvum induced B7-H1 expression in cultured human cholangiocytes. Induced expression of B7-H1 was identified in cells after exposure to infective C. parvum parasite or parasite lysate. Interestingly, the level of microRNA513 (miR-513) was reduced in cells after exposure to C. parvum, which resulted in a relief of 3' untranslated region-mediated translations suppression of B7-H1. Overexpression of miR-513 through transfection of miR513 precursor inhibited C. parvum-indaced B7-H1 protein expression. Moreover, enhanced apoptotic cell death was identified in activated human T cells after coculture with C. parvum-infected cholangiocytes. The apoptosis of activated T cells was partially blocked by a neutralizing antibody to B7-H1 or transfection of cholangiocytes with miR-513 precursor. These data suggest a role of miR-513 in regulating B7-H1 expression by cholangiocytes in response to C. parvum infection.

Original languageEnglish (US)
Pages (from-to)160-169
Number of pages10
JournalJournal of Infectious Diseases
Issue number1
StatePublished - Jan 2010

ASJC Scopus subject areas

  • Immunology and Allergy
  • Infectious Diseases


Dive into the research topics of 'Cryptosporidium parvum induces B7-H1 expression in cholangiocytes by down-regulating microRNA-513'. Together they form a unique fingerprint.

Cite this