Comparative Transcriptomic Analysis of Staphylococcus aureus Associated with Periprosthetic Joint Infection under in Vivo and in Vitro Conditions

Thao Le Masters, Stephen Johnson, Patricio R. Jeraldo, Kerryl E. Greenwood-Quaintance, Scott A. Cunningham, Matthew P. Abdel, Nicholas Chia, Robin Patel

Research output: Contribution to journalArticlepeer-review

Abstract

Transcriptomic analysis can provide insight as to how Staphylococcus aureus adapts to the environmental niche of periprosthetic joint infection (PJI), a challenging clinical infection. Here, in vivo RNA expression of eight S. aureus PJIs was compared with expression of the corresponding isolates in planktonic culture using a total RNA-sequencing approach. Expression varied among isolates, with a common trend showing increased expression of several ica-independent biofilm formation genes, including sdr, fnb, ebpS, and aaa; genes encoding enzymes and toxins, including coa, nuc, hlb, and hlgA/B/C; and genes facilitating acquisition of iron via the iron-binding molecule siderophore B (snb) and heme consumption protein (isd) pathways in PJI. Several antimicrobial resistance determinants were detected; although their presence correlated with phenotypic susceptibility of the associated isolates, no difference in expression between in vivo and in vitro conditions was identified.

Original languageEnglish (US)
Pages (from-to)986-999
Number of pages14
JournalJournal of Molecular Diagnostics
Volume23
Issue number8
DOIs
StatePublished - Aug 2021

ASJC Scopus subject areas

  • Pathology and Forensic Medicine
  • Molecular Medicine

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