TY - JOUR
T1 - Comparative Transcriptomic Analysis of Staphylococcus aureus Associated with Periprosthetic Joint Infection under in Vivo and in Vitro Conditions
AU - Le Masters, Thao
AU - Johnson, Stephen
AU - Jeraldo, Patricio R.
AU - Greenwood-Quaintance, Kerryl E.
AU - Cunningham, Scott A.
AU - Abdel, Matthew P.
AU - Chia, Nicholas
AU - Patel, Robin
N1 - Publisher Copyright:
© 2021 Association for Molecular Pathology and American Society for Investigative Pathology
PY - 2021/8
Y1 - 2021/8
N2 - Transcriptomic analysis can provide insight as to how Staphylococcus aureus adapts to the environmental niche of periprosthetic joint infection (PJI), a challenging clinical infection. Here, in vivo RNA expression of eight S. aureus PJIs was compared with expression of the corresponding isolates in planktonic culture using a total RNA-sequencing approach. Expression varied among isolates, with a common trend showing increased expression of several ica-independent biofilm formation genes, including sdr, fnb, ebpS, and aaa; genes encoding enzymes and toxins, including coa, nuc, hlb, and hlgA/B/C; and genes facilitating acquisition of iron via the iron-binding molecule siderophore B (snb) and heme consumption protein (isd) pathways in PJI. Several antimicrobial resistance determinants were detected; although their presence correlated with phenotypic susceptibility of the associated isolates, no difference in expression between in vivo and in vitro conditions was identified.
AB - Transcriptomic analysis can provide insight as to how Staphylococcus aureus adapts to the environmental niche of periprosthetic joint infection (PJI), a challenging clinical infection. Here, in vivo RNA expression of eight S. aureus PJIs was compared with expression of the corresponding isolates in planktonic culture using a total RNA-sequencing approach. Expression varied among isolates, with a common trend showing increased expression of several ica-independent biofilm formation genes, including sdr, fnb, ebpS, and aaa; genes encoding enzymes and toxins, including coa, nuc, hlb, and hlgA/B/C; and genes facilitating acquisition of iron via the iron-binding molecule siderophore B (snb) and heme consumption protein (isd) pathways in PJI. Several antimicrobial resistance determinants were detected; although their presence correlated with phenotypic susceptibility of the associated isolates, no difference in expression between in vivo and in vitro conditions was identified.
UR - http://www.scopus.com/inward/record.url?scp=85110272845&partnerID=8YFLogxK
UR - http://www.scopus.com/inward/citedby.url?scp=85110272845&partnerID=8YFLogxK
U2 - 10.1016/j.jmoldx.2021.05.011
DO - 10.1016/j.jmoldx.2021.05.011
M3 - Article
C2 - 34098085
AN - SCOPUS:85110272845
SN - 1525-1578
VL - 23
SP - 986
EP - 999
JO - Journal of Molecular Diagnostics
JF - Journal of Molecular Diagnostics
IS - 8
ER -