TY - JOUR
T1 - Comparative Transcriptomic Analysis of Staphylococcus aureus Associated with Periprosthetic Joint Infection under in Vivo and in Vitro Conditions
AU - Le Masters, Thao
AU - Johnson, Stephen
AU - Jeraldo, Patricio R.
AU - Greenwood-Quaintance, Kerryl E.
AU - Cunningham, Scott A.
AU - Abdel, Matthew P.
AU - Chia, Nicholas
AU - Patel, Robin
N1 - Funding Information:
Supported by the National Institute of Arthritis and Musculoskeletal and Skin Diseases of the NIH under grants R01 AR056647 (R.P.) and T32 AR56950 (Musculoskeletal Research Training Program, T.L.M.).Disclosures: R.P. reports grants from CD Diagnostics, Merck, Hutchison Biofilm Medical Solutions, Accelerate Diagnostics, ContraFect, TenNor Therapeutics Limited, and Shionogi. R.P. is a consultant to Curetis, Specific Technologies, NextGen Diagnostics, PathoQuest, Selux Diagnostics, and Qvella; monies are paid to Mayo Clinic. In addition, R.P. has a patent on Bordetella pertussis/parapertussis PCR, a patent on a device/method for sonication with royalties paid by Samsung to the Mayo Clinic, and a patent on an anti-biofilm substance. R.P. receives travel reimbursement from the American Society for Microbiology and the Infectious Diseases Society of America, an editor's stipend from the Infectious Diseases Society of America, and honoraria from the NBME, UpToDate, and the Infectious Diseases Board Review Course. M.P.A. receives royalties from Stryker on certain hip and knee products and is a paid consultant for Stryker.
Funding Information:
Supported by the National Institute of Arthritis and Musculoskeletal and Skin Diseases of the NIH under grants R01 AR056647 (R.P.) and T32 AR56950 (Musculoskeletal Research Training Program, T.L.M.).
Funding Information:
Disclosures: R.P. reports grants from CD Diagnostics , Merck , Hutchison Biofilm Medical Solutions , Accelerate Diagnostics , ContraFect , TenNor Therapeutics Limited , and Shionogi . R.P. is a consultant to Curetis, Specific Technologies, NextGen Diagnostics, PathoQuest, Selux Diagnostics, and Qvella; monies are paid to Mayo Clinic. In addition, R.P. has a patent on Bordetella pertussis/parapertussis PCR, a patent on a device/method for sonication with royalties paid by Samsung to the Mayo Clinic, and a patent on an anti-biofilm substance. R.P. receives travel reimbursement from the American Society for Microbiology and the Infectious Diseases Society of America, an editor’s stipend from the Infectious Diseases Society of America, and honoraria from the NBME, UpToDate, and the Infectious Diseases Board Review Course. M.P.A. receives royalties from Stryker on certain hip and knee products and is a paid consultant for Stryker.
Publisher Copyright:
© 2021 Association for Molecular Pathology and American Society for Investigative Pathology
PY - 2021/8
Y1 - 2021/8
N2 - Transcriptomic analysis can provide insight as to how Staphylococcus aureus adapts to the environmental niche of periprosthetic joint infection (PJI), a challenging clinical infection. Here, in vivo RNA expression of eight S. aureus PJIs was compared with expression of the corresponding isolates in planktonic culture using a total RNA-sequencing approach. Expression varied among isolates, with a common trend showing increased expression of several ica-independent biofilm formation genes, including sdr, fnb, ebpS, and aaa; genes encoding enzymes and toxins, including coa, nuc, hlb, and hlgA/B/C; and genes facilitating acquisition of iron via the iron-binding molecule siderophore B (snb) and heme consumption protein (isd) pathways in PJI. Several antimicrobial resistance determinants were detected; although their presence correlated with phenotypic susceptibility of the associated isolates, no difference in expression between in vivo and in vitro conditions was identified.
AB - Transcriptomic analysis can provide insight as to how Staphylococcus aureus adapts to the environmental niche of periprosthetic joint infection (PJI), a challenging clinical infection. Here, in vivo RNA expression of eight S. aureus PJIs was compared with expression of the corresponding isolates in planktonic culture using a total RNA-sequencing approach. Expression varied among isolates, with a common trend showing increased expression of several ica-independent biofilm formation genes, including sdr, fnb, ebpS, and aaa; genes encoding enzymes and toxins, including coa, nuc, hlb, and hlgA/B/C; and genes facilitating acquisition of iron via the iron-binding molecule siderophore B (snb) and heme consumption protein (isd) pathways in PJI. Several antimicrobial resistance determinants were detected; although their presence correlated with phenotypic susceptibility of the associated isolates, no difference in expression between in vivo and in vitro conditions was identified.
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U2 - 10.1016/j.jmoldx.2021.05.011
DO - 10.1016/j.jmoldx.2021.05.011
M3 - Article
C2 - 34098085
AN - SCOPUS:85110272845
SN - 1525-1578
VL - 23
SP - 986
EP - 999
JO - Journal of Molecular Diagnostics
JF - Journal of Molecular Diagnostics
IS - 8
ER -