Collagen gel contraction as a measure of fibroblast function in carpal tunnel syndrome

Tai Hua Yang, Andrew R. Thoreson, Anne Gingery, Kai Nan An, Dirk R. Larson, Chunfeng Zhao, Peter C. Amadio

Research output: Contribution to journalArticlepeer-review

8 Scopus citations


Noninflammatory subsynovial connective tissue (SSCT) fibrosis with nerve compression is a prominent feature of carpal tunnel syndrome (CTS). Studies have shown that SSCT matrix synthesis and material property changes in CTS are associated with increased activity of transforming growth factor (TGF)-β1. The aim of this study were to (1) investigate the ability of SSCT fibroblasts from CTS patients and unaffected individuals to contract a collagen gel ring and (2) determine how the addition of TGF-β1 affects this ability. SSCT fibroblasts from three normal cadavers and three age-matched female patients who had undergone surgery for CTS were used. Results showed patient cell-seeded gels had a significantly higher contraction rate (p < 0.001) than control cells, and fully contracted gel rings possessed a significantly higher tensile strength (p = 0.003) and stiffness (p < 0.001). Furthermore, TGF-β1 significantly intensified contraction rate (p < 0.001), tensile strength (p < 0.001), and stiffness (p < 0.001). In conclusion, SSCT cells from normal donors and CTS patients contract collagen gel rings differently, and this ability is affected by TGF-β1 treatment. This cell-seeded collagen gel model may be useful for developing new methods of stopping or eliminating the effect of TGF-β1 on the SSCT fibroblasts and surrounding matrix, which might aid in the identification of medical treatment for CTS.

Original languageEnglish (US)
Pages (from-to)574-580
Number of pages7
JournalJournal of Biomedical Materials Research - Part A
Issue number2
StatePublished - Feb 1 2015


  • TGF-β1
  • carpal tunnel syndrome
  • collagen gel contraction
  • fibrosis
  • subsynovial connective tissue

ASJC Scopus subject areas

  • Ceramics and Composites
  • Biomaterials
  • Biomedical Engineering
  • Metals and Alloys


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