Cloning, expression and characterization of the human NOB1 gene

Yue Zhang; Jun Ni; Guangjin Zhou; Jian Yuan; Weihua Ren; Yuxi Shan; Wenwen Tang; Long Yu; Shouyuan Zhao

doi:10.1007/s11033-005-3141-7

Cloning, expression and characterization of the human NOB1 gene

Yue Zhang, Jun Ni, Guangjin Zhou, Jian Yuan, Weihua Ren, Yuxi Shan, Wenwen Tang, Long Yu, Shouyuan Zhao

Pharmacology

Research output: Contribution to journal › Article › peer-review

37 Scopus citations

Abstract

The yeast Nob1p (Nin one binding protein) gene is required for proteasome function and RNA metabolism. We report here the cloning and characterization of the human orthologue NOB1 gene and its products. The human NOB1 gene is composed of nine exons and eight introns and is localized on human chromosome 16q22.1. The NOB1 cDNA is 1749 bp long and contains a putative open reading frame of 1239 bp. The predicted NOB1 protein comprises a PIN (PilT amino terminus) domain and a zinc ribbon domain. Western blot analysis showed that the molecular weight of NOB1 is about 50 KDa. RT-PCR analysis of mRNA from human adult tissues showed that NOB1 is expressed mainly in liver, lung and spleen. Expression of NOB1 in mammalian culture cells indicated that the NOB1 protein is mainly localized in the nucleus. Our data provides important information for further study of the function of the NOB1 gene and its products.

Original language	English (US)
Pages (from-to)	185-189
Number of pages	5
Journal	Molecular Biology Reports
Volume	32
Issue number	3
DOIs	https://doi.org/10.1007/s11033-005-3141-7
State	Published - Sep 2005

Keywords

NOB1
PIN domain
Subcellular localization
Tissue expression pattern
Zinc ribbon domain

ASJC Scopus subject areas

Molecular Biology
Genetics

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10.1007/s11033-005-3141-7

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title = "Cloning, expression and characterization of the human NOB1 gene",

abstract = "The yeast Nob1p (Nin one binding protein) gene is required for proteasome function and RNA metabolism. We report here the cloning and characterization of the human orthologue NOB1 gene and its products. The human NOB1 gene is composed of nine exons and eight introns and is localized on human chromosome 16q22.1. The NOB1 cDNA is 1749 bp long and contains a putative open reading frame of 1239 bp. The predicted NOB1 protein comprises a PIN (PilT amino terminus) domain and a zinc ribbon domain. Western blot analysis showed that the molecular weight of NOB1 is about 50 KDa. RT-PCR analysis of mRNA from human adult tissues showed that NOB1 is expressed mainly in liver, lung and spleen. Expression of NOB1 in mammalian culture cells indicated that the NOB1 protein is mainly localized in the nucleus. Our data provides important information for further study of the function of the NOB1 gene and its products.",

keywords = "NOB1, PIN domain, Subcellular localization, Tissue expression pattern, Zinc ribbon domain",

author = "Yue Zhang and Jun Ni and Guangjin Zhou and Jian Yuan and Weihua Ren and Yuxi Shan and Wenwen Tang and Long Yu and Shouyuan Zhao",

note = "Funding Information: We are very grateful to Dr. Qiang Yu (Boston University) for a critical reading of the manuscript. This work was supported by the National 973 program of China, 863 projects of China and the National Natural Science foundation of China (30024001).",

year = "2005",

month = sep,

doi = "10.1007/s11033-005-3141-7",

language = "English (US)",

volume = "32",

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T1 - Cloning, expression and characterization of the human NOB1 gene

AU - Zhang, Yue

AU - Ni, Jun

AU - Zhou, Guangjin

AU - Yuan, Jian

AU - Ren, Weihua

AU - Shan, Yuxi

AU - Tang, Wenwen

AU - Yu, Long

AU - Zhao, Shouyuan

N1 - Funding Information: We are very grateful to Dr. Qiang Yu (Boston University) for a critical reading of the manuscript. This work was supported by the National 973 program of China, 863 projects of China and the National Natural Science foundation of China (30024001).

PY - 2005/9

Y1 - 2005/9

N2 - The yeast Nob1p (Nin one binding protein) gene is required for proteasome function and RNA metabolism. We report here the cloning and characterization of the human orthologue NOB1 gene and its products. The human NOB1 gene is composed of nine exons and eight introns and is localized on human chromosome 16q22.1. The NOB1 cDNA is 1749 bp long and contains a putative open reading frame of 1239 bp. The predicted NOB1 protein comprises a PIN (PilT amino terminus) domain and a zinc ribbon domain. Western blot analysis showed that the molecular weight of NOB1 is about 50 KDa. RT-PCR analysis of mRNA from human adult tissues showed that NOB1 is expressed mainly in liver, lung and spleen. Expression of NOB1 in mammalian culture cells indicated that the NOB1 protein is mainly localized in the nucleus. Our data provides important information for further study of the function of the NOB1 gene and its products.

AB - The yeast Nob1p (Nin one binding protein) gene is required for proteasome function and RNA metabolism. We report here the cloning and characterization of the human orthologue NOB1 gene and its products. The human NOB1 gene is composed of nine exons and eight introns and is localized on human chromosome 16q22.1. The NOB1 cDNA is 1749 bp long and contains a putative open reading frame of 1239 bp. The predicted NOB1 protein comprises a PIN (PilT amino terminus) domain and a zinc ribbon domain. Western blot analysis showed that the molecular weight of NOB1 is about 50 KDa. RT-PCR analysis of mRNA from human adult tissues showed that NOB1 is expressed mainly in liver, lung and spleen. Expression of NOB1 in mammalian culture cells indicated that the NOB1 protein is mainly localized in the nucleus. Our data provides important information for further study of the function of the NOB1 gene and its products.

KW - NOB1

KW - PIN domain

KW - Subcellular localization

KW - Tissue expression pattern

KW - Zinc ribbon domain

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Cloning, expression and characterization of the human NOB1 gene

Abstract

Keywords

ASJC Scopus subject areas

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