Cloning and characterization of the CDZFP gene which encodes a putative zinc finger protein

Wenwen Tang; Jian Yuan; Xinya Chen; Yuxi Shan; Kuntian Luo; Zekun Guo; Yue Zhang; Bo Wan; Long Yu

doi:10.1080/10425170500224305

Cloning and characterization of the CDZFP gene which encodes a putative zinc finger protein

Wenwen Tang, Jian Yuan, Xinya Chen, Yuxi Shan, Kuntian Luo, Zekun Guo, Yue Zhang, Bo Wan, Long Yu

Pharmacology

Research output: Contribution to journal › Article › peer-review

4 Scopus citations

Abstract

We report here the cloning and characterization of a novel human cytoplasm-distribution zinc finger protein (CDZFP) gene, isolated from human ovary cDNA library, and mapped to 4p12 by searching the UCSC genomic database. The CDZFP cDNA is 1793 base pairs in length and contains an open reading frame (ORF) encoding 236 amino acids. The CDZFP gene consists of 7 exons and encodes a putative zinc finger protein with a transmembrane region and two zinc finger motifs. Subcellular localization demonstrated that CDZFP protein was located in the cytoplasm when overexpressed in Hela cells and northern blot analysis revealed that CDZFP was ubiquitously expressed in 16 human tissues.

Original language	English (US)
Pages (from-to)	391-396
Number of pages	6
Journal	DNA Sequence - Journal of DNA Sequencing and Mapping
Volume	16
Issue number	5
DOIs	https://doi.org/10.1080/10425170500224305
State	Published - Oct 1 2005

Keywords

CDZFP
DNA binding
Northern blot
Subcellular localization

ASJC Scopus subject areas

Biochemistry
Molecular Biology
Genetics
Endocrinology

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10.1080/10425170500224305

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title = "Cloning and characterization of the CDZFP gene which encodes a putative zinc finger protein",

abstract = "We report here the cloning and characterization of a novel human cytoplasm-distribution zinc finger protein (CDZFP) gene, isolated from human ovary cDNA library, and mapped to 4p12 by searching the UCSC genomic database. The CDZFP cDNA is 1793 base pairs in length and contains an open reading frame (ORF) encoding 236 amino acids. The CDZFP gene consists of 7 exons and encodes a putative zinc finger protein with a transmembrane region and two zinc finger motifs. Subcellular localization demonstrated that CDZFP protein was located in the cytoplasm when overexpressed in Hela cells and northern blot analysis revealed that CDZFP was ubiquitously expressed in 16 human tissues.",

keywords = "CDZFP, DNA binding, Northern blot, Subcellular localization",

author = "Wenwen Tang and Jian Yuan and Xinya Chen and Yuxi Shan and Kuntian Luo and Zekun Guo and Yue Zhang and Bo Wan and Long Yu",

note = "Funding Information: We are grateful to Drs Songmin Jiang and Daojun Jiang for their revising this paper and helpful discussions. We also thank Drs Guangqing Cao for the gene sequencing. This work was supported by the National 973 program of China, 863 projects of China and the National Natural Science foundation of China (30024001).",

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doi = "10.1080/10425170500224305",

language = "English (US)",

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journal = "DNA Sequence - Journal of DNA Sequencing and Mapping",

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T1 - Cloning and characterization of the CDZFP gene which encodes a putative zinc finger protein

AU - Tang, Wenwen

AU - Yuan, Jian

AU - Chen, Xinya

AU - Shan, Yuxi

AU - Luo, Kuntian

AU - Guo, Zekun

AU - Zhang, Yue

AU - Wan, Bo

AU - Yu, Long

N1 - Funding Information: We are grateful to Drs Songmin Jiang and Daojun Jiang for their revising this paper and helpful discussions. We also thank Drs Guangqing Cao for the gene sequencing. This work was supported by the National 973 program of China, 863 projects of China and the National Natural Science foundation of China (30024001).

PY - 2005/10/1

Y1 - 2005/10/1

N2 - We report here the cloning and characterization of a novel human cytoplasm-distribution zinc finger protein (CDZFP) gene, isolated from human ovary cDNA library, and mapped to 4p12 by searching the UCSC genomic database. The CDZFP cDNA is 1793 base pairs in length and contains an open reading frame (ORF) encoding 236 amino acids. The CDZFP gene consists of 7 exons and encodes a putative zinc finger protein with a transmembrane region and two zinc finger motifs. Subcellular localization demonstrated that CDZFP protein was located in the cytoplasm when overexpressed in Hela cells and northern blot analysis revealed that CDZFP was ubiquitously expressed in 16 human tissues.

AB - We report here the cloning and characterization of a novel human cytoplasm-distribution zinc finger protein (CDZFP) gene, isolated from human ovary cDNA library, and mapped to 4p12 by searching the UCSC genomic database. The CDZFP cDNA is 1793 base pairs in length and contains an open reading frame (ORF) encoding 236 amino acids. The CDZFP gene consists of 7 exons and encodes a putative zinc finger protein with a transmembrane region and two zinc finger motifs. Subcellular localization demonstrated that CDZFP protein was located in the cytoplasm when overexpressed in Hela cells and northern blot analysis revealed that CDZFP was ubiquitously expressed in 16 human tissues.

KW - CDZFP

KW - DNA binding

KW - Northern blot

KW - Subcellular localization

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Cloning and characterization of the CDZFP gene which encodes a putative zinc finger protein

Abstract

Keywords

ASJC Scopus subject areas

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