Abstract
Leucine specific tRNA of soybean cotyledons was frationated into six peaks (1-6). The relative amounts of Leu-tRNA 5 and 6 are lower in developing cotyledons than in germinating cotyledons. Leu-tRNA synthetase from developing cotyledons is less active in aminoacylating Leu-tRNA 5 and 6 compared to enzyme from 5-day-old germinating cotyledons. Leu-tRNA synthetase from cotyledons of germinating seedlings and developing cotyledons can be fractionated into three peaks (1-3). Peak 1 in the developing cotyledon is about 36% less than peak 1 from 5-day-old germinating cotyledons. Peaks 2 and 3 from developing cotyledons are about 10 and 18% higher than from germinating cotyledons, respectively. Peak 1 from developing cotyledons acylates all six species of Leu-tRNA in contrast with peak 1 from germinating cotyledons, which essentially acylates only Leu-tRNA 5 and 6. The specificity of peaks 2 and 3 towards Leu-tRNA 1-4 is identical in both the organs.
| Original language | English (US) |
|---|---|
| Pages (from-to) | 1809-1812 |
| Number of pages | 4 |
| Journal | Phytochemistry |
| Volume | 15 |
| Issue number | 12 |
| DOIs | |
| State | Published - 1976 |
Keywords
- Glycine max
- Leguminosae
- hydroxylapatite column
- leucyl transfer RNA synthetase
- leucyl, transfer RNAs
- reversed phase chromatography.
- soybean
ASJC Scopus subject areas
- Biochemistry
- Molecular Biology
- Plant Science
- Horticulture