Boosting of SARS-CoV-2 immunity in nonhuman primates using an oral rhabdoviral vaccine

Kah Whye Peng, Timothy Carey, Patrycja Lech, Rianna Vandergaast, Miguel Muñoz-Alía, Nandakumar Packiriswamy, Clement Gnanadurai, Karina Krotova, Mulu Tesfay, Christopher Ziegler, Michelle Haselton, Kara Sevola, Chase Lathrum, Samantha Reiter, Riya Narjari, Baskar Balakrishnan, Lukkana Suksanpaisan, Toshie Sakuma, Jordan Recker, Lianwen ZhangScott Waniger, Luke Russell, Christopher D. Petro, Christos A. Kyratsous, Alina Baum, Jody L. Janecek, Rachael M. Lee, Sabarinathan Ramachandran, Melanie L. Graham, Stephen J. Russell

Research output: Contribution to journalArticlepeer-review


An orally active vaccine capable of boosting SARS-CoV-2 immune responses in previously infected or vaccinated individuals would help efforts to achieve and sustain herd immunity. Unlike mRNA-loaded lipid nanoparticles and recombinant replication-defective adenoviruses, replicating vesicular stomatitis viruses with SARS-CoV-2 spike glycoproteins (VSV-SARS2) were poorly immunogenic after intramuscular administration in clinical trials. Here, by G protein trans-complementation, we generated VSV-SARS2(+G) virions with expanded target cell tropism. Compared to parental VSV-SARS2, G-supplemented viruses were orally active in virus-naive and vaccine-primed cynomolgus macaques, powerfully boosting SARS-CoV-2 neutralizing antibody titers. Clinical testing of this oral VSV-SARS2(+G) vaccine is planned.

Original languageEnglish (US)
Pages (from-to)2342-2351
Number of pages10
Issue number15
StatePublished - Apr 1 2022


  • Neutralizing antibodies
  • Oral vaccine
  • Rhabdovirus
  • SARS-CoV-2
  • T cells
  • VSV

ASJC Scopus subject areas

  • Molecular Medicine
  • General Immunology and Microbiology
  • General Veterinary
  • Public Health, Environmental and Occupational Health
  • Infectious Diseases


Dive into the research topics of 'Boosting of SARS-CoV-2 immunity in nonhuman primates using an oral rhabdoviral vaccine'. Together they form a unique fingerprint.

Cite this