Biochemical characterization of the pancreatic cholecystokinin receptor: A possible marker of cell differentiation and development

L. J. Miller; S. P. Powers

doi:10.3109/00365528809095921

Biochemical characterization of the pancreatic cholecystokinin receptor: A possible marker of cell differentiation and development

L. J. Miller, S. P. Powers

Gastroenterology and Hepatology

Research output: Contribution to journal › Article › peer-review

1 Scopus citations

Abstract

The biochemical expression of the cholecystokinin (CCK) receptor on the surface of the pancreatic acinar cell is a potential marker of the state of differentiation of that cell. In this report we review the basis for and the results of the use of affinity labeling techniques for the biochemical characterization of this macromolecular receptor assembly on the adult rat pancreatic acinar cell. A series of specially designed molecular probes are used to define the subunit structure of this receptor, based on the relationships between the sites of covalent attachment of these probes and their receptor-binding domains. We suggest that the receptor-binding domain resides on a M_r = 85.000-95.000 subunit. whereas a distinct M_r = 80.000 also exists as part of this complex.

Original language	English (US)
Pages (from-to)	104-107
Number of pages	4
Journal	Scandinavian Journal of Gastroenterology
Volume	23
Issue number	S151
DOIs	https://doi.org/10.3109/00365528809095921
State	Published - 1988

Keywords

Affinity labeling
Cholecystokinin receptor
Differentiation

ASJC Scopus subject areas

Gastroenterology

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10.3109/00365528809095921

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title = "Biochemical characterization of the pancreatic cholecystokinin receptor: A possible marker of cell differentiation and development",

abstract = "The biochemical expression of the cholecystokinin (CCK) receptor on the surface of the pancreatic acinar cell is a potential marker of the state of differentiation of that cell. In this report we review the basis for and the results of the use of affinity labeling techniques for the biochemical characterization of this macromolecular receptor assembly on the adult rat pancreatic acinar cell. A series of specially designed molecular probes are used to define the subunit structure of this receptor, based on the relationships between the sites of covalent attachment of these probes and their receptor-binding domains. We suggest that the receptor-binding domain resides on a Mr = 85.000-95.000 subunit. whereas a distinct Mr = 80.000 also exists as part of this complex.",

keywords = "Affinity labeling, Cholecystokinin receptor, Differentiation",

author = "Miller, {L. J.} and Powers, {S. P.}",

note = "Funding Information: We wish to acknowledge the assistance of Marilyn LeQve in preparing this manuscript. This work was supported by grants DK32878 and DK34988 from the National Institutes of Health and by the American Gastroenterological Association-Upjohn Research Scholar Award.",

year = "1988",

doi = "10.3109/00365528809095921",

language = "English (US)",

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pages = "104--107",

journal = "Scandinavian Journal of Gastroenterology",

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T1 - Biochemical characterization of the pancreatic cholecystokinin receptor

T2 - A possible marker of cell differentiation and development

AU - Miller, L. J.

AU - Powers, S. P.

N1 - Funding Information: We wish to acknowledge the assistance of Marilyn LeQve in preparing this manuscript. This work was supported by grants DK32878 and DK34988 from the National Institutes of Health and by the American Gastroenterological Association-Upjohn Research Scholar Award.

PY - 1988

Y1 - 1988

N2 - The biochemical expression of the cholecystokinin (CCK) receptor on the surface of the pancreatic acinar cell is a potential marker of the state of differentiation of that cell. In this report we review the basis for and the results of the use of affinity labeling techniques for the biochemical characterization of this macromolecular receptor assembly on the adult rat pancreatic acinar cell. A series of specially designed molecular probes are used to define the subunit structure of this receptor, based on the relationships between the sites of covalent attachment of these probes and their receptor-binding domains. We suggest that the receptor-binding domain resides on a Mr = 85.000-95.000 subunit. whereas a distinct Mr = 80.000 also exists as part of this complex.

AB - The biochemical expression of the cholecystokinin (CCK) receptor on the surface of the pancreatic acinar cell is a potential marker of the state of differentiation of that cell. In this report we review the basis for and the results of the use of affinity labeling techniques for the biochemical characterization of this macromolecular receptor assembly on the adult rat pancreatic acinar cell. A series of specially designed molecular probes are used to define the subunit structure of this receptor, based on the relationships between the sites of covalent attachment of these probes and their receptor-binding domains. We suggest that the receptor-binding domain resides on a Mr = 85.000-95.000 subunit. whereas a distinct Mr = 80.000 also exists as part of this complex.

KW - Affinity labeling

KW - Cholecystokinin receptor

KW - Differentiation

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JF - Scandinavian Journal of Gastroenterology

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Biochemical characterization of the pancreatic cholecystokinin receptor: A possible marker of cell differentiation and development

Abstract

Keywords

ASJC Scopus subject areas

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