Atractylodes japonica suppresses lipopolysaccharide-stimulated expressions of inducible nitric oxide synthase and cyclooxygenase-2 in RAW 264.7 macrophages

Mi Hyeon Jang; Min Chul Shin; Ye Jin Kim; Chang Ju Kim; Yeunhee Kim; Ee Hwa Kim

doi:10.1248/bpb.27.324

Atractylodes japonica suppresses lipopolysaccharide-stimulated expressions of inducible nitric oxide synthase and cyclooxygenase-2 in RAW 264.7 macrophages

Mi Hyeon Jang, Min Chul Shin, Ye Jin Kim, Chang Ju Kim, Yeunhee Kim, Ee Hwa Kim

Neurosurgery

Research output: Contribution to journal › Article › peer-review

21 Scopus citations

Abstract

Atractylodes japonica has traditionally been used for the treatment of pain and arthritis. The effect of Atractylodes japonica against lipopolysaccharide- induced inflammation was investigated using reverse transcription-polymerase chain reaction (RT-PCR), nitric oxide detection, and prostaglandin E₂ (PGE₂) immunoassay in mouse RAW 264.7 macrophages. The aqueous extract of Atractylodes japonica suppressed nitric oxide production and PGE ₂ synthesis by inhibition of the lipopolysaccharide-stimulated enhancement of inducible nitric oxide synthase and cyclooxygenase-2 mRNAs expressions in RAW 264.7 macrophages. These results suggest that Atractylodes japonica exerts anti-inflammatory and analgesic effects probably by suppression of the inducible nitric oxide synthase and cyclooxygenase-2 expressions.

Original language	English (US)
Pages (from-to)	324-327
Number of pages	4
Journal	Biological and Pharmaceutical Bulletin
Volume	27
Issue number	3
DOIs	https://doi.org/10.1248/bpb.27.324
State	Published - Mar 2004

Keywords

Atractylodes japonica
Cyclooxygenase-2
Inducible nitric oxide synthase
Lipopolysaccharide

ASJC Scopus subject areas

Pharmacology
Pharmaceutical Science

Access to Document

10.1248/bpb.27.324

Cite this

@article{2d823b5705cb4b7b96e515ee80395ab5,

title = "Atractylodes japonica suppresses lipopolysaccharide-stimulated expressions of inducible nitric oxide synthase and cyclooxygenase-2 in RAW 264.7 macrophages",

abstract = "Atractylodes japonica has traditionally been used for the treatment of pain and arthritis. The effect of Atractylodes japonica against lipopolysaccharide- induced inflammation was investigated using reverse transcription-polymerase chain reaction (RT-PCR), nitric oxide detection, and prostaglandin E2 (PGE2) immunoassay in mouse RAW 264.7 macrophages. The aqueous extract of Atractylodes japonica suppressed nitric oxide production and PGE 2 synthesis by inhibition of the lipopolysaccharide-stimulated enhancement of inducible nitric oxide synthase and cyclooxygenase-2 mRNAs expressions in RAW 264.7 macrophages. These results suggest that Atractylodes japonica exerts anti-inflammatory and analgesic effects probably by suppression of the inducible nitric oxide synthase and cyclooxygenase-2 expressions.",

keywords = "Atractylodes japonica, Cyclooxygenase-2, Inducible nitric oxide synthase, Lipopolysaccharide",

author = "Jang, {Mi Hyeon} and Shin, {Min Chul} and Kim, {Ye Jin} and Kim, {Chang Ju} and Yeunhee Kim and Kim, {Ee Hwa}",

year = "2004",

month = mar,

doi = "10.1248/bpb.27.324",

language = "English (US)",

volume = "27",

pages = "324--327",

journal = "Biological and Pharmaceutical Bulletin",

issn = "0918-6158",

publisher = "Pharmaceutical Society of Japan",

number = "3",

}

TY - JOUR

T1 - Atractylodes japonica suppresses lipopolysaccharide-stimulated expressions of inducible nitric oxide synthase and cyclooxygenase-2 in RAW 264.7 macrophages

AU - Jang, Mi Hyeon

AU - Shin, Min Chul

AU - Kim, Ye Jin

AU - Kim, Chang Ju

AU - Kim, Yeunhee

AU - Kim, Ee Hwa

PY - 2004/3

Y1 - 2004/3

N2 - Atractylodes japonica has traditionally been used for the treatment of pain and arthritis. The effect of Atractylodes japonica against lipopolysaccharide- induced inflammation was investigated using reverse transcription-polymerase chain reaction (RT-PCR), nitric oxide detection, and prostaglandin E2 (PGE2) immunoassay in mouse RAW 264.7 macrophages. The aqueous extract of Atractylodes japonica suppressed nitric oxide production and PGE 2 synthesis by inhibition of the lipopolysaccharide-stimulated enhancement of inducible nitric oxide synthase and cyclooxygenase-2 mRNAs expressions in RAW 264.7 macrophages. These results suggest that Atractylodes japonica exerts anti-inflammatory and analgesic effects probably by suppression of the inducible nitric oxide synthase and cyclooxygenase-2 expressions.

AB - Atractylodes japonica has traditionally been used for the treatment of pain and arthritis. The effect of Atractylodes japonica against lipopolysaccharide- induced inflammation was investigated using reverse transcription-polymerase chain reaction (RT-PCR), nitric oxide detection, and prostaglandin E2 (PGE2) immunoassay in mouse RAW 264.7 macrophages. The aqueous extract of Atractylodes japonica suppressed nitric oxide production and PGE 2 synthesis by inhibition of the lipopolysaccharide-stimulated enhancement of inducible nitric oxide synthase and cyclooxygenase-2 mRNAs expressions in RAW 264.7 macrophages. These results suggest that Atractylodes japonica exerts anti-inflammatory and analgesic effects probably by suppression of the inducible nitric oxide synthase and cyclooxygenase-2 expressions.

KW - Atractylodes japonica

KW - Cyclooxygenase-2

KW - Inducible nitric oxide synthase

KW - Lipopolysaccharide

UR - http://www.scopus.com/inward/record.url?scp=16544392335&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=16544392335&partnerID=8YFLogxK

U2 - 10.1248/bpb.27.324

DO - 10.1248/bpb.27.324

M3 - Article

C2 - 14993796

AN - SCOPUS:16544392335

SN - 0918-6158

VL - 27

SP - 324

EP - 327

JO - Biological and Pharmaceutical Bulletin

JF - Biological and Pharmaceutical Bulletin

IS - 3

ER -

Atractylodes japonica suppresses lipopolysaccharide-stimulated expressions of inducible nitric oxide synthase and cyclooxygenase-2 in RAW 264.7 macrophages

Abstract

Keywords

ASJC Scopus subject areas

Access to Document

Other files and links

Fingerprint

Cite this