Assessing immune function by profiling cytokine release from stimulated blood leukocytes and the risk of infection in rheumatoid arthritis

Megan L. Krause; John M. Davis; Keith L. Knutson; Michael A. Strausbach; Cynthia S. Crowson; Terry M. Therneau; Peter J. Wettstein; Eric L. Matteson; Sherine E. Gabriel

doi:10.1016/j.clim.2011.05.008

Assessing immune function by profiling cytokine release from stimulated blood leukocytes and the risk of infection in rheumatoid arthritis

Megan L. Krause, John M. Davis, Keith L. Knutson, Michael A. Strausbach, Cynthia S. Crowson, Terry M. Therneau, Peter J. Wettstein, Eric L. Matteson, Sherine E. Gabriel

Research output: Contribution to journal › Article › peer-review

8 Scopus citations

Abstract

Persons with rheumatoid arthritis (RA) suffer a high burden of infections, but currently no biomarkers are available to identify individuals at greatest risk. A prospective longitudinal study was therefore conducted to determine the association between the responsiveness of ex vivo cytokine production and 6-month risk of infections. Infections were identified by billing codes and validated by medical record review. At baseline, the release of 17 cytokines by peripheral blood mononuclear cells in response to stimulation, or media alone, was measured using multiplexed cytokine analysis. Production of IL-2, IL-8, IL-10, IL-17, TNF-α, IFN-β, and GM-CSF, induced by various conditions, was significantly associated with the occurrence of infections. A multivariable prediction model based on these data provided new information on the risk of infection beyond standard assessments of disease activity, severity, and treatment. Future studies could utilize this information to devise new biomarkers for the prediction of infection in patients with RA.

Original language	English (US)
Pages (from-to)	67-72
Number of pages	6
Journal	Clinical Immunology
Volume	141
Issue number	1
DOIs	https://doi.org/10.1016/j.clim.2011.05.008
State	Published - Oct 2011

Keywords

Immune response
Immune signature
Peripheral blood mononuclear cells

ASJC Scopus subject areas

Immunology and Allergy
Immunology

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10.1016/j.clim.2011.05.008

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Krause, M. L., Davis, J. M., Knutson, K. L., Strausbach, M. A., Crowson, C. S., Therneau, T. M., Wettstein, P. J., Matteson, E. L., & Gabriel, S. E. (2011). Assessing immune function by profiling cytokine release from stimulated blood leukocytes and the risk of infection in rheumatoid arthritis. Clinical Immunology, 141(1), 67-72. https://doi.org/10.1016/j.clim.2011.05.008

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title = "Assessing immune function by profiling cytokine release from stimulated blood leukocytes and the risk of infection in rheumatoid arthritis",

abstract = "Persons with rheumatoid arthritis (RA) suffer a high burden of infections, but currently no biomarkers are available to identify individuals at greatest risk. A prospective longitudinal study was therefore conducted to determine the association between the responsiveness of ex vivo cytokine production and 6-month risk of infections. Infections were identified by billing codes and validated by medical record review. At baseline, the release of 17 cytokines by peripheral blood mononuclear cells in response to stimulation, or media alone, was measured using multiplexed cytokine analysis. Production of IL-2, IL-8, IL-10, IL-17, TNF-α, IFN-β, and GM-CSF, induced by various conditions, was significantly associated with the occurrence of infections. A multivariable prediction model based on these data provided new information on the risk of infection beyond standard assessments of disease activity, severity, and treatment. Future studies could utilize this information to devise new biomarkers for the prediction of infection in patients with RA.",

keywords = "Immune response, Immune signature, Peripheral blood mononuclear cells",

author = "Krause, {Megan L.} and Davis, {John M.} and Knutson, {Keith L.} and Strausbach, {Michael A.} and Crowson, {Cynthia S.} and Therneau, {Terry M.} and Wettstein, {Peter J.} and Matteson, {Eric L.} and Gabriel, {Sherine E.}",

note = "Funding Information: This project was supported by NIH/NIAMS grant number R01 R46849 , NIH/NCRR CTSA grant number UL1 RR024150 , and by NIH/NCRR CTSA grant number KL2 RR024151 . The contents of this publication are solely the responsibility of the authors and do not necessarily represent the official views of the NIH. The funding sources had no involvement in the study design; in the collection, analysis, or interpretation of data; in the writing of this report; or in the decision to submit the paper for publication. ",

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doi = "10.1016/j.clim.2011.05.008",

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Assessing immune function by profiling cytokine release from stimulated blood leukocytes and the risk of infection in rheumatoid arthritis

Abstract

Keywords

ASJC Scopus subject areas

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