Transgenic expression of the RNA-dependent RNA polymerase 3Dpol inhibited infection of Theiler's murine encephalitis virus (TMEV), a picornavirus from which it was derived. Here, we infected 3Dpol transgenic mice with another picornavirus, as well as an alphaherpesvirus and a rhabdovirus. 3Dpol transgenic FVB mice had significantly lower viral loads and survived longer after infection with all three types of viruses than nontransgenic FVB mice. Viral inhibition among three different types of virus by transgenic 3Dpol suggests that the mechanism of action is not the direct interference with picornaviral 3Dpol but instead may be the changing of host cells to an antiviral state before or after viral infection occurs, as basal interferon levels were higher in 3Dpol transgenic mice before infection. Further study of this mechanism may open new possibilities for future antiviral therapy.
ASJC Scopus subject areas
- Insect Science