Analysis of protein phosphorylation using mass spectrometry: Deciphering the phosphoproteome

Matthias Mann, Shao En Ong, Mads Grønborg, Hanno Steen, Ole N. Jensen, Akhilesh Pandey

Research output: Contribution to journalReview articlepeer-review

755 Scopus citations


In signal transduction in eukaryotes, protein phosphorylation is a key event. To understand signaling processes, we must first acquire an inventory of phosphoproteins and their phosphorylation sites under different conditions. Because phosphorylation is a dynamic process, elucidation of signaling networks also requires quantitation of these phosphorylation events. In this article, we outline several methods for enrichment of phosphorylated proteins and peptides and discuss various options for their identification and quantitation with special emphasis on mass spectrometry-based techniques.

Original languageEnglish (US)
Pages (from-to)261-268
Number of pages8
JournalTrends in Biotechnology
Issue number6
StatePublished - Jun 1 2002

ASJC Scopus subject areas

  • Biotechnology
  • Bioengineering


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