@inbook{1029f78f541e4afb82d5653e0560351e,
title = "Analysis of CYLD proteolysis by CASPASE 8 in bone marrow-derived macrophages",
abstract = "Previous studies have demonstrated that CASPASE 8 can generate a prosurvival signal by inhibiting necroptosis via the cleavage of the deubiquitinating enzyme CYLD. Cleavage of CYLD at D215 results in the generation of a 25 kD N-terminal fragment and degradation of the C-terminal fragment containing the catalytic domain. Since CYLD is required for TNF-induced necroptosis, its proteolysis is necessary and sufficient to suppress necroptosis and generate a survival signal. Here we describe how to visualize CYLD proteolysis by western blot analysis, as a measure of CASPASE 8 activity and inhibition of necroptosis.",
keywords = "Bone marrow-derived macrophages, CYLD, Cleavage, Necroptosis, Proteolysis",
author = "Diana Legarda and Ting, \{Adrian T.\}",
note = "Publisher Copyright: {\textcopyright} 2018, Springer Science+Business Media, LLC, part of Springer Nature.",
year = "2018",
doi = "10.1007/978-1-4939-8754-2\_18",
language = "English (US)",
series = "Methods in Molecular Biology",
publisher = "Humana Press Inc.",
pages = "181--188",
booktitle = "Methods in Molecular Biology",
}