Amino-terminal domain of NF1 binds to DNA as a dimer and activates adenovirus DNA replication

Fotini Gounari, Raffaele De Francesco, Jacky Schmitt, Peter C. Van Der Vliet, Riccardo Cortese, Henk Stunnenberg

Research output: Contribution to journalArticlepeer-review


NF1 is a DNA-binding protein involved in initiation of adenovirus DNA replication as well as in modulating the rate of transcription initiation of genes containing the sequence TGGCA. We show here that recombinant NF1 expressed via vaccinia virus is transported into the nucleus and binds to its cognate sequences with the same specificity as NF1 purified from HeLa cells. Furthermore, the recombinant NF1 forms oligomers in solution and binds as a dimer to palindromic as well as half-site sequences. NF1 expressed via vaccinia virus stimulates the initiation of adenovirus replication in vitro. The N-terminal 240 amino acids of the protein are sufficient for full DNA-binding activity as well as stimulation of adenovirus replication. By analysis of several NF1 mutants translated in vitro, we also define the minimal DNA-binding domain and localize the region responsible for DNA binding on the N-terminal and for oligomerization on the C-terminal side of this domain.

Original languageEnglish (US)
Pages (from-to)559-566
Number of pages8
JournalEMBO Journal
Issue number2
StatePublished - 1990


  • Adenovirus
  • DNA replication
  • NF1
  • Oligomerization

ASJC Scopus subject areas

  • General Immunology and Microbiology
  • General Biochemistry, Genetics and Molecular Biology
  • Molecular Biology
  • General Neuroscience


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