@article{e4786f291f1f47bd9da309e8f827292d,
title = "Alzheimer's disease: Early alterations in brain DNA methylation at ANK1, BIN1, RHBDF2 and other loci",
abstract = "We used a collection of 708 prospectively collected autopsied brains to assess the methylation state of the brain's DNA in relation to Alzheimer's disease (AD). We found that the level of methylation at 71 of the 415,848 interrogated CpGs was significantly associated with the burden of AD pathology, including CpGs in the ABCA7 and BIN1 regions, which harbor known AD susceptibility variants. We validated 11 of the differentially methylated regions in an independent set of 117 subjects. Furthermore, we functionally validated these CpG associations and identified the nearby genes whose RNA expression was altered in AD: ANK1, CDH23, DIP2A, RHBDF2, RPL13, SERPINF1 and SERPINF2. Our analyses suggest that these DNA methylation changes may have a role in the onset of AD given that we observed them in presymptomatic subjects and that six of the validated genes connect to a known AD susceptibility gene network.",
author = "{De Jager}, {Philip L.} and Gyan Srivastava and Katie Lunnon and Jeremy Burgess and Schalkwyk, {Leonard C.} and Lei Yu and Eaton, {Matthew L.} and Keenan, {Brendan T.} and Jason Ernst and Cristin McCabe and Anna Tang and Towfique Raj and Joseph Replogle and Wendy Brodeur and Stacey Gabriel and Chai, {High S.} and Curtis Younkin and Younkin, {Steven G.} and Fanggeng Zou and Moshe Szyf and Epstein, {Charles B.} and Schneider, {Julie A.} and Bernstein, {Bradley E.} and Alex Meissner and Nilufer Ertekin-Taner and Chibnik, {Lori B.} and Manolis Kellis and Jonathan Mill and Bennett, {David A.}",
note = "Funding Information: We thank the National Institute for Health (NIHR) Biomedical Research Unit in Dementia in the South London and Maudsley NHS Foundation Trust (SLaM), Brains for Dementia Research (Alzheimer Brain Bank UK) and the donors and families who made this research possible. We also would like to thank the participants of the ROS and MAP studies for their participation in these studies. Support for this research was provided by grants from the US National Institutes of Health (R01 AG036042, R01AG036836, R01 AG17917,R01AG15819, R01 AG032990, R01 AG18023, RC2 AG036547, P30 AG10161, P50 AG016574, U01 ES017155, KL2 RR024151, K25 AG041906-01). Support was also provided by the Siragusa Foundation to N.E.-T., and the Robert and Clarice Smith and Abigail Van Buren Alzheimer{\textquoteright}s Disease Research Program to N.E.-T., S.G.Y. and F.Z. This work was funded by US National Institutes of Health grant AG036039 to J.M. and an Equipment Grant from Alzheimer{\textquoteright}s Research UK. Publisher Copyright: {\textcopyright} 2014 Nature America, Inc. All rights reserved.",
year = "2014",
month = sep,
day = "1",
doi = "10.1038/nn.3786",
language = "English (US)",
volume = "17",
pages = "1156--1163",
journal = "Nature Neuroscience",
issn = "1097-6256",
publisher = "Nature Publishing Group",
number = "9",
}