Alteration of cell membrane by stress waves in vitro

Shun Lee, Therese Anderson, Hong Zhang, Thomas J. Flotte, Apostolos G. Doukas

Research output: Contribution to journalArticlepeer-review

95 Scopus citations


Experiments on the biological effects of laser-induced stress waves indicate that there is a transient increase in the permeability of the cell membrane. A cell viability assay (propidium iodide exclusion) shows that mouse breast sarcoma cells are viable after a stress wave. The kinetics of this transient membrane permeability are measured using time-resolved fluorescence imaging. The efflux of a membrane-impermeable fluorescent probe (calcein) following the application of a 300-bar stress wave implies that there is an increase in the membrane permeability. This efflux ceases within 80 s after a stress wave, suggesting that the membrane is no longer permeable to the fluorescent probe. Fitting the observed kinetics to a simple diffusion model yields an average initial diffusion constant of 2.2 ± 1.3 x 10-7 cm2/s for mouse breast sarcoma cells following the application of a laser- induced stress wave.

Original languageEnglish (US)
Pages (from-to)1285-1293
Number of pages9
JournalUltrasound in Medicine and Biology
Issue number9
StatePublished - 1996


  • Diffusion
  • Membrane permeability
  • Molecular delivery
  • Photoacoustics
  • Shock waves
  • Time resolved fluorescence imaging

ASJC Scopus subject areas

  • Biophysics
  • Radiological and Ultrasound Technology
  • Acoustics and Ultrasonics


Dive into the research topics of 'Alteration of cell membrane by stress waves in vitro'. Together they form a unique fingerprint.

Cite this