TY - JOUR
T1 - Alkylacylglycerol molecular species in the glycosylinositol phospholipid membrane anchor of bovine erythrocyte acetylcholinesterase
AU - Roberts, William L.
AU - Myher, John J.
AU - Kuksis, Arnis
AU - Rosenberry, Terrone L.
N1 - Funding Information:
and by grants from the Muscular Council of Canada and the Heart
PY - 1988/1/15
Y1 - 1988/1/15
N2 - Bovine erythrocyte acetylcholinesterase, a glycosylinositol phospholipid anchored membrane enzyme, was digested with phosphatidylinositol-specific phospholipase C and the released glycerol-containing moieties were identified and quantitated. About 96% of the total was alkylacylglycerol, of which sn-1-stearyl-2-stearoylglycerol, sn-1-stearyl-2-oleoylglycerol andsn-1-oleyl-2-stearoylglycerol accounted for 69%, 13% and 10%, respectively. These alkylacylglycerols are in marked contrast to the exclusively diacylglycerol species present in phosphatidylinositol from bovine erythrocyte membranes. This difference suggests that assembly of the membrane anchor of Ebo AChE involves a selected cellular pool of diradylglycerols.
AB - Bovine erythrocyte acetylcholinesterase, a glycosylinositol phospholipid anchored membrane enzyme, was digested with phosphatidylinositol-specific phospholipase C and the released glycerol-containing moieties were identified and quantitated. About 96% of the total was alkylacylglycerol, of which sn-1-stearyl-2-stearoylglycerol, sn-1-stearyl-2-oleoylglycerol andsn-1-oleyl-2-stearoylglycerol accounted for 69%, 13% and 10%, respectively. These alkylacylglycerols are in marked contrast to the exclusively diacylglycerol species present in phosphatidylinositol from bovine erythrocyte membranes. This difference suggests that assembly of the membrane anchor of Ebo AChE involves a selected cellular pool of diradylglycerols.
UR - http://www.scopus.com/inward/record.url?scp=0023837629&partnerID=8YFLogxK
UR - http://www.scopus.com/inward/citedby.url?scp=0023837629&partnerID=8YFLogxK
U2 - 10.1016/0006-291X(88)90516-5
DO - 10.1016/0006-291X(88)90516-5
M3 - Article
C2 - 3337715
AN - SCOPUS:0023837629
SN - 0006-291X
VL - 150
SP - 271
EP - 277
JO - Biochemical and Biophysical Research Communications
JF - Biochemical and Biophysical Research Communications
IS - 1
ER -