Adipose tissue DNA methylome changes in development of new-onset diabetes after kidney transplantation

Saurabh Baheti, Prachi Singh, Yun Zhang, Jared Evans, Michael D. Jensen, Virend K. Somers, Jean Pierre A. Kocher, Zhifu Sun, Harini A. Chakkera

Research output: Contribution to journalArticlepeer-review

4 Scopus citations


Aim: New-onset diabetes after kidney transplant (NODAT) adversely impacts kidney allograft and patient survival. Epigenetic alterations in adipose tissue like DNA methylation may play a contributory role. Methods: Adipose tissue DNA of the patients with NODAT and their age, sex and BMI matched controls (nine each) were sequenced by reduced representation bisulfite sequencing. Differentially methylated CpGs (DMCs) and differentially methylated regions (DMRs) were studied. Results: Adipose tissue from the patients had reduced DNA methylation in intergenic and intronic regions. DMCs were found to be more hypomethylated in repeat regions and hypermethylated in CGIs and promoter region. About 900 DMRs were found and their associated genes were significantly enriched in 32 pathways, the top ones of which were associated with insulin resistance and inflammation. Some DMR or DMC genes have known T2DM associations. Conclusion: Changes in DNA methylation in adipose tissue may be suggestive of future NODAT.

Original languageEnglish (US)
Pages (from-to)1423-1435
Number of pages13
Issue number11
StatePublished - Nov 2017


  • DNA methylation
  • RRBS
  • adipose tissue
  • new-onset diabetes after transplant

ASJC Scopus subject areas

  • Genetics
  • Cancer Research


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