@article{b8ecc59c73b34f85b1e84930f85e692a,
title = "Activity of a hypochlorous acid-producing electrochemical bandage as assessed with a porcine explant biofilm model",
abstract = "The activity of a hypochlorous acid-producing electrochemical bandage (e-bandage) in preventing methicillin-resistant Staphylococcus aureus infection (MRSA) infection and removing biofilms formed by MRSA was assessed using a porcine explant biofilm model. e-Bandages inhibited S. aureus infection (p = 0.029) after 12 h (h) of exposure and reduced 3-day biofilm viable cell counts after 6, 12, and 24 h exposures (p = 0.029). Needle-type microelectrodes were used to assess HOCl concentrations in explant tissue as a result of e-bandage treatment; toxicity associated with e-bandage treatment was evaluated. HOCl concentrations in infected and uninfected explant tissue varied between 30 and 80 µM, decreasing with increasing distance from the e-bandage. Eukaryotic cell viability was reduced by an average of 71% and 65% in fresh and day 3-old explants, respectively, when compared to explants exposed to nonpolarized e-bandages. HOCl e-bandages are a promising technology that can be further developed as an antibiotic-free treatment for wound biofilm infections.",
keywords = "MRSA, Staphylococcus aureus, biofilms, chronic wounds, electrochemical bandage, hypochlorous acid",
author = "Gretchen Tibbits and Abdelrhman Mohamed and Suzanne Gelston and Laure Flurin and Raval, {Yash S.} and Greenwood-Quaintance, {Kerryl E.} and Robin Patel and Haluk Beyenal",
note = "Funding Information: Research reported in this publication was supported by the National Institute of Allergy and Infectious Diseases of the National Institutes of Health under award number R01 AI091594. The content is solely the responsibility of the authors and does not necessarily represent the official views of the National Institutes of Health. The authors thank C&L Locker in Moscow, ID, the University of Idaho Beef Center, and the Washington State University Premium Beef for providing the porcine materials. Electron microscopy, sample preparation protocols, and support were provided by the Washington State University Franceschi Microscopy and Imaging Center. Authors also like to thank Md Monzurul Islam Anoy for his suggestions on the manuscript. Funding Information: Research reported in this publication was supported by the National Institute of Allergy and Infectious Diseases of the National Institutes of Health under award number R01 AI091594. The content is solely the responsibility of the authors and does not necessarily represent the official views of the National Institutes of Health. The authors thank C&L Locker in Moscow, ID, the University of Idaho Beef Center, and the Washington State University Premium Beef for providing the porcine materials. Electron microscopy, sample preparation protocols, and support were provided by the Washington State University Franceschi Microscopy and Imaging Center. Authors also like to thank Md Monzurul Islam Anoy for his suggestions on the manuscript. Publisher Copyright: {\textcopyright} 2022 The Authors. Biotechnology and Bioengineering published by Wiley Periodicals LLC.",
year = "2023",
month = jan,
doi = "10.1002/bit.28248",
language = "English (US)",
volume = "120",
pages = "250--259",
journal = "Biotechnology and Bioengineering",
issn = "0006-3592",
publisher = "Wiley-VCH Verlag",
number = "1",
}