A Tissue Engineered Construct for Laryngeal Regeneration: A Proof-of-Concept Device Design Study

David G. Lott, Manisha Shah, Cheryl Myers, Michael McPhail, Juergen Neubauer, Jason Struthers, Cathy S. Madsen, Danielle Grandjean, Stephanie R.C. Zacharias, Yourka D. Tchoukalova

Research output: Contribution to journalArticlepeer-review


Objectives/Hypothesis: Develop a patient-specific tissue engineered construct for laryngeal reconstruction following a partial laryngectomy. Study Design: Bench and animal research. Methods: A construct made from a porous polyethylene scaffold shaped in a canine-specific configuration and seeded with autologous canine adipose-derived stem cells in fibrin glue was implanted in a canine following a partial laryngectomy. After 1 year, the construct was first evaluated in vivo with high-speed imaging and acoustic–aerodynamic measures. It was then explanted and evaluated histologically. Results: The canine study at 1 year revealed the construct provided voicing (barking) with acoustic and aerodynamic measures within normal ranges. The canine was able to eat and breathe normally without long-term support. The construct was integrated with epithelialization of all areas except the medial portion of the vocal fold structure. No anti-infective agents were needed after the standard perioperative medications were completed. Conclusion: This study provided a successful first step toward developing a patient-specific composite construct for patients undergoing partial laryngectomies. Level of Evidence: NA Laryngoscope, 132:S1–S11, 2022.

Original languageEnglish (US)
Pages (from-to)S1-S11
Issue numberS9
StatePublished - Jun 2022


  • Tissue engineering
  • airway reconstruction
  • epithelialization
  • partial laryngectomy

ASJC Scopus subject areas

  • Otorhinolaryngology


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