A scalable workflow to characterize the human exposome

Xin Hu; Douglas I. Walker; Yongliang Liang; Matthew Ryan Smith; Michael L. Orr; Brian D. Juran; Chunyu Ma; Karan Uppal; Michael Koval; Greg S. Martin; David C. Neujahr; Carmen J. Marsit; Young Mi Go; Kurt D. Pennell; Gary W. Miller; Konstantinos N. Lazaridis; Dean P. Jones

doi:10.1038/s41467-021-25840-9

A scalable workflow to characterize the human exposome

Xin Hu, Douglas I. Walker, Yongliang Liang, Matthew Ryan Smith, Michael L. Orr, Brian D. Juran, Chunyu Ma, Karan Uppal, Michael Koval, Greg S. Martin, David C. Neujahr, Carmen J. Marsit, Young Mi Go, Kurt D. Pennell, Gary W. Miller, Konstantinos N. Lazaridis, Dean P. Jones

Gastroenterology and Hepatology

Research output: Contribution to journal › Article › peer-review

Abstract

Complementing the genome with an understanding of the human exposome is an important challenge for contemporary science and technology. Tens of thousands of chemicals are used in commerce, yet cost for targeted environmental chemical analysis limits surveillance to a few hundred known hazards. To overcome limitations which prevent scaling to thousands of chemicals, we develop a single-step express liquid extraction and gas chromatography high-resolution mass spectrometry analysis to operationalize the human exposome. We show that the workflow supports quantification of environmental chemicals in human plasma (200 µL) and tissue (≤100 mg) samples. The method also provides high resolution, sensitivity and selectivity for exposome epidemiology of mass spectral features without a priori knowledge of chemical identity. The simplicity of the method can facilitate harmonization of environmental biomonitoring between laboratories and enable population level human exposome research with limited sample volume.

Original language	English (US)
Article number	5575
Journal	Nature communications
Volume	12
Issue number	1
DOIs	https://doi.org/10.1038/s41467-021-25840-9
State	Published - Dec 1 2021

ASJC Scopus subject areas

Chemistry(all)
Biochemistry, Genetics and Molecular Biology(all)
Physics and Astronomy(all)

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Hu, X., Walker, D. I., Liang, Y., Smith, M. R., Orr, M. L., Juran, B. D., Ma, C., Uppal, K., Koval, M., Martin, G. S., Neujahr, D. C., Marsit, C. J., Go, Y. M., Pennell, K. D., Miller, G. W., Lazaridis, K. N., & Jones, D. P. (2021). A scalable workflow to characterize the human exposome. Nature communications, 12(1), Article 5575. https://doi.org/10.1038/s41467-021-25840-9

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title = "A scalable workflow to characterize the human exposome",

abstract = "Complementing the genome with an understanding of the human exposome is an important challenge for contemporary science and technology. Tens of thousands of chemicals are used in commerce, yet cost for targeted environmental chemical analysis limits surveillance to a few hundred known hazards. To overcome limitations which prevent scaling to thousands of chemicals, we develop a single-step express liquid extraction and gas chromatography high-resolution mass spectrometry analysis to operationalize the human exposome. We show that the workflow supports quantification of environmental chemicals in human plasma (200 µL) and tissue (≤100 mg) samples. The method also provides high resolution, sensitivity and selectivity for exposome epidemiology of mass spectral features without a priori knowledge of chemical identity. The simplicity of the method can facilitate harmonization of environmental biomonitoring between laboratories and enable population level human exposome research with limited sample volume.",

author = "Xin Hu and Walker, {Douglas I.} and Yongliang Liang and Smith, {Matthew Ryan} and Orr, {Michael L.} and Juran, {Brian D.} and Chunyu Ma and Karan Uppal and Michael Koval and Martin, {Greg S.} and Neujahr, {David C.} and Marsit, {Carmen J.} and Go, {Young Mi} and Pennell, {Kurt D.} and Miller, {Gary W.} and Lazaridis, {Konstantinos N.} and Jones, {Dean P.}",

note = "Funding Information: This study was supported by the NIEHS, U2C ES030163 (DPJ), R24 ES029490 (DPJ, XH), U2C ES030859 (DIW) and P30 ES019776 (CJM), NIDDK RC2 DK118619 (KNL), NHLBI R01 HL086773 (DPJ), US Department of Defense W81XWH2010103 (DPJ), and the Chris M. Carlos and Catharine Nicole Jockisch Carlos Endowment Fund in Primary Sclerosing Cholangitis (PSC) (KNL). Funding Information: The raw data generated in this study have been deposited in the National Institutes of Health (NIH) Common Fund{\textquoteright}s National Metabolomics Data Repository (NMDR), the Metabolomics Workbench (https://www.metabolomicsworkbench.org) under assigned Project ID PR001136, accessible at https://doi.org/10.21228/M8VQ4D59. The Metabolomics Workbench is supported by NIH grant U2C-DK119886. Source data are provided with this paper. Publisher Copyright: {\textcopyright} 2021, The Author(s).",

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doi = "10.1038/s41467-021-25840-9",

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AU - Hu, Xin

AU - Walker, Douglas I.

AU - Liang, Yongliang

AU - Smith, Matthew Ryan

AU - Orr, Michael L.

AU - Juran, Brian D.

AU - Ma, Chunyu

AU - Uppal, Karan

AU - Koval, Michael

AU - Martin, Greg S.

AU - Neujahr, David C.

AU - Marsit, Carmen J.

AU - Go, Young Mi

AU - Pennell, Kurt D.

AU - Miller, Gary W.

AU - Lazaridis, Konstantinos N.

AU - Jones, Dean P.

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N2 - Complementing the genome with an understanding of the human exposome is an important challenge for contemporary science and technology. Tens of thousands of chemicals are used in commerce, yet cost for targeted environmental chemical analysis limits surveillance to a few hundred known hazards. To overcome limitations which prevent scaling to thousands of chemicals, we develop a single-step express liquid extraction and gas chromatography high-resolution mass spectrometry analysis to operationalize the human exposome. We show that the workflow supports quantification of environmental chemicals in human plasma (200 µL) and tissue (≤100 mg) samples. The method also provides high resolution, sensitivity and selectivity for exposome epidemiology of mass spectral features without a priori knowledge of chemical identity. The simplicity of the method can facilitate harmonization of environmental biomonitoring between laboratories and enable population level human exposome research with limited sample volume.

AB - Complementing the genome with an understanding of the human exposome is an important challenge for contemporary science and technology. Tens of thousands of chemicals are used in commerce, yet cost for targeted environmental chemical analysis limits surveillance to a few hundred known hazards. To overcome limitations which prevent scaling to thousands of chemicals, we develop a single-step express liquid extraction and gas chromatography high-resolution mass spectrometry analysis to operationalize the human exposome. We show that the workflow supports quantification of environmental chemicals in human plasma (200 µL) and tissue (≤100 mg) samples. The method also provides high resolution, sensitivity and selectivity for exposome epidemiology of mass spectral features without a priori knowledge of chemical identity. The simplicity of the method can facilitate harmonization of environmental biomonitoring between laboratories and enable population level human exposome research with limited sample volume.

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A scalable workflow to characterize the human exposome

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