A DIGITAL MICROFLUIDIC-BASED ELECTROCHEMICAL IMPEDANCE SPECTROSCOPY FOR CELL-BASED IMMUNOASSY IN A DYNAMIC MODE

Yuqian Zhang; Yuguang Liu

A DIGITAL MICROFLUIDIC-BASED ELECTROCHEMICAL IMPEDANCE SPECTROSCOPY FOR CELL-BASED IMMUNOASSY IN A DYNAMIC MODE

Surgery

Research output: Chapter in Book/Report/Conference proceeding › Conference contribution

Abstract

In this work, we report a novel system that integrates an electrochemical impedance spectroscopy (EIS)-based biosensor and a digital microfluidic (DMF) device. This system can quantify human peripheral blood mononuclear cells (PBMCs) in low-volume samples with minimal human intervention. By exploiting the unique droplet handling ability of DMF devices, we conducted a cell-based assay in a dynamic mode by agitating the sample droplet during the incubation, and detected as low as 10⁴ PBMCs/mL, enhancing sensitivity by 2.4-fold.

Original language	English (US)
Title of host publication	MicroTAS 2022 - 26th International Conference on Miniaturized Systems for Chemistry and Life Sciences
Publisher	Chemical and Biological Microsystems Society
Pages	130-131
Number of pages	2
ISBN (Electronic)	9781733419048
State	Published - 2022
Event	26th International Conference on Miniaturized Systems for Chemistry and Life Sciences, MicroTAS 2022 - Hybrid, Hangzhou, China Duration: Oct 23 2022 → Oct 27 2022

Publication series

Name	MicroTAS 2022 - 26th International Conference on Miniaturized Systems for Chemistry and Life Sciences

Conference

Conference	26th International Conference on Miniaturized Systems for Chemistry and Life Sciences, MicroTAS 2022
Country/Territory	China
City	Hybrid, Hangzhou
Period	10/23/22 → 10/27/22

Keywords

cell-based immunoassay
digital microfluidics
non-faradic electrochemical impedance spectroscopy (EIS)

ASJC Scopus subject areas

Chemical Engineering (miscellaneous)
Bioengineering
General Chemistry
Control and Systems Engineering

Cite this

Zhang, Y., & Liu, Y. (2022). A DIGITAL MICROFLUIDIC-BASED ELECTROCHEMICAL IMPEDANCE SPECTROSCOPY FOR CELL-BASED IMMUNOASSY IN A DYNAMIC MODE. In MicroTAS 2022 - 26th International Conference on Miniaturized Systems for Chemistry and Life Sciences (pp. 130-131). (MicroTAS 2022 - 26th International Conference on Miniaturized Systems for Chemistry and Life Sciences). Chemical and Biological Microsystems Society.

A DIGITAL MICROFLUIDIC-BASED ELECTROCHEMICAL IMPEDANCE SPECTROSCOPY FOR CELL-BASED IMMUNOASSY IN A DYNAMIC MODE. / Zhang, Yuqian; Liu, Yuguang.
MicroTAS 2022 - 26th International Conference on Miniaturized Systems for Chemistry and Life Sciences. Chemical and Biological Microsystems Society, 2022. p. 130-131 (MicroTAS 2022 - 26th International Conference on Miniaturized Systems for Chemistry and Life Sciences).

Research output: Chapter in Book/Report/Conference proceeding › Conference contribution

Zhang, Y & Liu, Y 2022, A DIGITAL MICROFLUIDIC-BASED ELECTROCHEMICAL IMPEDANCE SPECTROSCOPY FOR CELL-BASED IMMUNOASSY IN A DYNAMIC MODE. in MicroTAS 2022 - 26th International Conference on Miniaturized Systems for Chemistry and Life Sciences. MicroTAS 2022 - 26th International Conference on Miniaturized Systems for Chemistry and Life Sciences, Chemical and Biological Microsystems Society, pp. 130-131, 26th International Conference on Miniaturized Systems for Chemistry and Life Sciences, MicroTAS 2022, Hybrid, Hangzhou, China, 10/23/22.

Zhang Y, Liu Y. A DIGITAL MICROFLUIDIC-BASED ELECTROCHEMICAL IMPEDANCE SPECTROSCOPY FOR CELL-BASED IMMUNOASSY IN A DYNAMIC MODE. In MicroTAS 2022 - 26th International Conference on Miniaturized Systems for Chemistry and Life Sciences. Chemical and Biological Microsystems Society. 2022. p. 130-131. (MicroTAS 2022 - 26th International Conference on Miniaturized Systems for Chemistry and Life Sciences).

Zhang, Yuqian ; Liu, Yuguang. / A DIGITAL MICROFLUIDIC-BASED ELECTROCHEMICAL IMPEDANCE SPECTROSCOPY FOR CELL-BASED IMMUNOASSY IN A DYNAMIC MODE. MicroTAS 2022 - 26th International Conference on Miniaturized Systems for Chemistry and Life Sciences. Chemical and Biological Microsystems Society, 2022. pp. 130-131 (MicroTAS 2022 - 26th International Conference on Miniaturized Systems for Chemistry and Life Sciences).

@inproceedings{961a034f93da4c9ba54b4882dd7fa66f,

title = "A DIGITAL MICROFLUIDIC-BASED ELECTROCHEMICAL IMPEDANCE SPECTROSCOPY FOR CELL-BASED IMMUNOASSY IN A DYNAMIC MODE",

abstract = "In this work, we report a novel system that integrates an electrochemical impedance spectroscopy (EIS)-based biosensor and a digital microfluidic (DMF) device. This system can quantify human peripheral blood mononuclear cells (PBMCs) in low-volume samples with minimal human intervention. By exploiting the unique droplet handling ability of DMF devices, we conducted a cell-based assay in a dynamic mode by agitating the sample droplet during the incubation, and detected as low as 104 PBMCs/mL, enhancing sensitivity by 2.4-fold.",

keywords = "cell-based immunoassay, digital microfluidics, non-faradic electrochemical impedance spectroscopy (EIS)",

author = "Yuqian Zhang and Yuguang Liu",

note = "Publisher Copyright: {\textcopyright} 2022 MicroTAS 2022 - 26th International Conference on Miniaturized Systems for Chemistry and Life Sciences. All rights reserved.; 26th International Conference on Miniaturized Systems for Chemistry and Life Sciences, MicroTAS 2022 ; Conference date: 23-10-2022 Through 27-10-2022",

year = "2022",

language = "English (US)",

series = "MicroTAS 2022 - 26th International Conference on Miniaturized Systems for Chemistry and Life Sciences",

publisher = "Chemical and Biological Microsystems Society",

pages = "130--131",

booktitle = "MicroTAS 2022 - 26th International Conference on Miniaturized Systems for Chemistry and Life Sciences",

}

TY - GEN

T1 - A DIGITAL MICROFLUIDIC-BASED ELECTROCHEMICAL IMPEDANCE SPECTROSCOPY FOR CELL-BASED IMMUNOASSY IN A DYNAMIC MODE

AU - Zhang, Yuqian

AU - Liu, Yuguang

PY - 2022

Y1 - 2022

N2 - In this work, we report a novel system that integrates an electrochemical impedance spectroscopy (EIS)-based biosensor and a digital microfluidic (DMF) device. This system can quantify human peripheral blood mononuclear cells (PBMCs) in low-volume samples with minimal human intervention. By exploiting the unique droplet handling ability of DMF devices, we conducted a cell-based assay in a dynamic mode by agitating the sample droplet during the incubation, and detected as low as 104 PBMCs/mL, enhancing sensitivity by 2.4-fold.

AB - In this work, we report a novel system that integrates an electrochemical impedance spectroscopy (EIS)-based biosensor and a digital microfluidic (DMF) device. This system can quantify human peripheral blood mononuclear cells (PBMCs) in low-volume samples with minimal human intervention. By exploiting the unique droplet handling ability of DMF devices, we conducted a cell-based assay in a dynamic mode by agitating the sample droplet during the incubation, and detected as low as 104 PBMCs/mL, enhancing sensitivity by 2.4-fold.

KW - cell-based immunoassay

KW - digital microfluidics

KW - non-faradic electrochemical impedance spectroscopy (EIS)

UR - http://www.scopus.com/inward/record.url?scp=85175690849&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=85175690849&partnerID=8YFLogxK

M3 - Conference contribution

AN - SCOPUS:85175690849

T3 - MicroTAS 2022 - 26th International Conference on Miniaturized Systems for Chemistry and Life Sciences

SP - 130

EP - 131

BT - MicroTAS 2022 - 26th International Conference on Miniaturized Systems for Chemistry and Life Sciences

PB - Chemical and Biological Microsystems Society

T2 - 26th International Conference on Miniaturized Systems for Chemistry and Life Sciences, MicroTAS 2022

Y2 - 23 October 2022 through 27 October 2022

ER -

A DIGITAL MICROFLUIDIC-BASED ELECTROCHEMICAL IMPEDANCE SPECTROSCOPY FOR CELL-BASED IMMUNOASSY IN A DYNAMIC MODE

Abstract

Publication series

Conference

Keywords

ASJC Scopus subject areas

Other files and links

Fingerprint

Cite this