Optimization of antigen preparations from Coccidioides for diagnostic testing

NARRATIVE Valley Fever is a fungal disease that is estimated to cause > 300,000 infections each year, yet the standard diagnostic approach is using serologic tests that are based on extracts of the soil- dwelling form of the fungus, rather than the human-disease form. The goal of this this research proposal is to extend our findings from the first published Coccidioides proteome to systematically improve antigen preparations used for serologic diagnosis. The primary goal of the project is to increase sensitivity in terms of detecting more individuals who truly have acute disease, and earlier diagnosis in their disease presentation to optimize their care, including avoiding unnecessary antibacterial drugs and administering antifungals when indicated.